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BL
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| Record Number
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668364
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dltt
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| Title & Author
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Technical aspects of toxicological immunohistochemistry : : system specified biomarkers /\ Syed A. Aziz, Rekha Mehta, editors
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| Page. NO
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1 online resource
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| ISBN
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9781493915163
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: 1493915169
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9781493915156
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1493915150
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| Notes
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Includes index
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| Contents
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Preface; Contents; Chapter 1: Immunohistochemistry: Overview, Its Potential, and Challenges; Overview; The Potential of IHC; Challenges; Conclusion; References; Chapter 2: Tissue Microarray Technology and Its Potential Applications in Toxicology and Toxicological Immunohistochemistry; Introduction; The Emergence and Dominance of Post-genomic Technologies; The Proliferation of Microarray Technologies; Tissue Microarrays; What Are TMAs?; Why Use TMAs?; How Is TMAs Produced?; TMA Resources; CHTN TMAs; The Tissue Array Research Program; National Disease Research Interchange
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Commercially Produced TMAs Bioinformatic Approaches for TMA Data Analysis; TMAs for Toxicological Research: Opportunities and Challenges; Applications in Toxicology; Conclusions; References; Chapter 3: Importance of Fluorescent In Situ Hybridization in Rodent Tumors; Introduction; Fluorescent In Situ Hybridization: A Conceptual Approach; DNA Probes; Single-Copy Probes; Whole-Genome Analysis in a Single Experiment with FISH-Based Technologies; Multiplex-FISH and Spectral Karyotyping Analysis; Comparative Genomic Hybridization Analyses; Microarrays Based Cytogenetics
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Multicolor Banding Analysis of Centromeres and Telomeres in Tumors; Combining Approaches: An Improvement in Cytogenetic Characterization; RNA Analysis in Single Cells; Concluding Remarks; References; Chapter 4: Quantitation of Immunohistochemistry by Image Analysis Technique; Introduction; Tissue Microarrays; Tissue-Based Diagnosis and Types of Diagnoses; Basics of Image Analysis; Principles of Microscopic Image Analysis; Colour Space; Image Quality; Object Segmentation; Syntactic Structure Algorithms; Image Information and Feature Extraction; Region of Interest
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Application in IHC Perspectives; References; Chapter 5: Immunohistochemistry in the Study of Cancer Biomarkers for Oncology Drug Development; Introduction; Technical Aspects of IHC; Basic Procedure of IHC; Fixation; FFPE Tissue Section and Frozen Fresh Tissue Cryosection; Tissue Pretreatment and Antigen Retrieval; Blocking Considerations; Primary Antibody and Controls; Detection Systems; Direct Methods; Indirect Method; Combination of Detection Systems for Multiple Target Protein Detection; Chromagens; Counterstaining; Coverslip; IHC for Cancer Biomarker Studies
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Cancer Biomarker Studies on Tissue Section Slides Cancer Biomarker Study on Cytological Preparation Slides; Assay Development; General Assay Development and Validation; Assay Development of Multi-fluorophore Labeling Techniques; Tumor Vascular Measurement; Circulating Tumor Cell Assessment; IHC Score; Score for Tumor Vasculature; Quantification of Microvessels and Target Receptors; Quantification of Hypoxic Protein Expression; Quantification of Pericyte Coverage; Score for Circulating Tumor Cells; Statistical Analysis; Companion Diagnostics; Sources of IHC Errors and Troubleshooting
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| Abstract
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This volume examines immunohistochemical methods for investigating the toxicologic pathology of rodent and otheranimal tissues.Eleven comprehensive chapters provide pathologists and researchers in various sub-disciplines of toxicology with an authoritative review of the methods ofimmunohistochemical staining in various target tissues. Further, this book explores the tissue-, antigen-, and antibody-specific problems that may be encountered during the staining procedures and provides potential avenues for resolving various methodological issues. Special attention is paid to the latest enhancement procedures for antigen retrieval and visualization as well as to image analysis and antigen quantification. Written by leading researchers in toxicology and pathology, this book is a significant resource for toxicologists and pathologists working with rodents and other animal models. The information in Technical Aspects of Toxicological Immunohistochemistry is rounded out bythe companion book,Toxicological Immunohistochemistrywhich examines mechanistic and predictive biomarkers for toxicological evaluation.
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| Subject
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Immunohistochemistry.
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Immunotoxicology.
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| Dewey Classification
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616.07/56
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| LC Classification
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QR183.6
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| Added Entry
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Aziz, Syed A.
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Mehta, Rekha
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| Added Entry
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Ohio Library and Information Network.
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