| Document Type
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BL
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| Record Number
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1002777
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| Doc. No
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b757147
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| Main Entry
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Likhtenshteĭn, G. I., (Gert︠s︡ Ilʹich)
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| Title & Author
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Biophysical labeling methods in molecular biology /\ Gertz I. Likhtenshtein.
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| Publication Statement
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Cambridge [England] ;New York, NY, USA :: Cambridge University Press,, 1993.
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| Page. NO
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1 online resource (xiv, 305 pages :: illustrations)
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| ISBN
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0511629095
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: 0511881789
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: 9780511629099
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: 9780511881787
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0521431328
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9780521017879 (paperback)
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9780521431323
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| Bibliographies/Indexes
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Includes bibliographical references (pages 266-302) and index.
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| Contents
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(Cont) Spin-labeled, physiologically active compounds -- Cells, tissues, organisms -- Biological assays -- Biological analyses.
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(Cont) Study of slow translational diffusion: photobleaching and fluctuation techniques -- Triplet labeling methods -- Peculiarities of triplet excited states -- Structures and chemical properties of triplet probes -- Exchange interactions with participation of excited triplet states: elements of theory -- Static exchange: experimental data -- Dynamic exchange processes -- Photochrome probes -- Triplet probe-photochrome labeling method -- Mossbauer spectroscopy, electron scattering and other labeling methods -- Mossbauer labels -- NMR probes -- Total tritium labeling technique -- Electron-scattering labels -- Studies of proteins and enzymes: structure, dynamics and mechanism of action -- Active centers of enzymes -- Conformational changes in proteins and enzymes -- Molecular dynamic properties of proteins and enzymes -- Physical labeling as a tool for studying the electron transfer mechanism -- Structure and dynamics of membranes -- Model membranes -- Biological membranes -- Nucleic acids and other biological systems: biological assays -- Nucleic acids -- Polysaccharides.
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Method of spin labeling -- Structure of nitroxide labels and probes -- ESR signals of NRs: magnetic parameters -- Methods of measurement of the ESR signal parameters -- Rotational diffusion of nitroxides -- Nitroxides as dielectric, pH, and redox probes -- Nitroxides in ESR tomography -- Spin traps -- Double-labeling techniques -- Effects of spin-spin interactions on the parameters of ESR spectra -- Determination of the distance between spins -- Spin label-spin probe method -- Nuclear magnetic resonance of paramagnetic systems -- Fluorescent labeling methods -- Chemical properties of fluorescent labels and probes -- Rotational diffusion of fluorescent chromophores -- Fluorescence and molecular dynamics of the medium -- Study of local acidity and electrostatic and polar properties of biological objects -- Inductive resonance energy transfer as a method of investigating structures and dynamics of biological objects -- Dynamic quenching of fluorescence as an approach to the study of molecular dynamics -- Charge transfer complexes, excimers and exciplexes as luminescent probes.
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| Abstract
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Physical labels such as stable nitroxide radicals, luminescent and photochromic chromophores, so-called Mössbauer atoms and electron-dense assemblies of heavy atoms have proved to be effective tools in solving many problems at the molecular level in biological systems. These physical labels are used as 'molecular rulers' to measure the distances between chosen groups and to measure the size, form and microrelief of objects. By providing information about these factors, the label provides information that can help the scientist to understand the structure of membranes, nucleic acids, enzymes and proteins and how they function. This 1993 volume covers all aspects of this field: the theoretical bases, the experimental techniques, and it also shows how to interpret the resulting data. It also critically discusses some recent results obtained with these techniques and gives an analysis of likely developments in the future.
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| Subject
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Biophysical labeling.
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Molecular biology-- Technique.
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Biologie moléculaire-- Technique.
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Biophysical labeling.
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Marqueurs biologiques.
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Marqueurs de spin.
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Molecular biology-- Technique.
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Sondes moléculaires.
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Electron Spin Resonance Spectroscopy-- methods.
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Spin Labels.
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Molecular Biology-- methods.
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| Dewey Classification
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574.8/8
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| LC Classification
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QH324.9.B49L55 1993
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| NLM classification
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1993 A-773
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QH 324.9.B49L727b 1993
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KN 12blsrissc
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