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" Lentivirus gene engineering protocols / "
edited by Maurizio Federico.
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BL
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| Record Number
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1003066
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b757436
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| Title & Author
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Lentivirus gene engineering protocols /\ edited by Maurizio Federico.
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| Publication Statement
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Totowa, N.J. :: Humana Press,, 2003.
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| Series Statement
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Methods in molecular biology ;; v. 229
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| Page. NO
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1 online resource (xi, 314 pages) :: illustrations
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| ISBN
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1280842687
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: 1588290913
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: 1592593933
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: 661084268X
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: 9781280842689
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: 9781588290915
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: 9781592593934
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: 9786610842681
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9781617373510
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| Bibliographies/Indexes
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Includes bibliographical references and index.
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| Contents
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From lentiviruses to lentivirus vectors / M. Federico -- The choice of a suitable lentivirus vector: Transcriptional targeting / F. Lotti and F. Mavilio -- Choice and use of appropriate packaging cell types / A.L. Pacchia, S. Mukherjee and J.P. Dougherty -- Small- to large-scale production of lentivirus vectors / M.P. Marino, M.J. Luce and J. Reiser -- Detection and titration of lentivirus vector preparations / J. Gatlin, M. Islas-Ohlmayer and J.V. Garcia -- Detection and selection of lentiviral vector-transduced cells / Y. Cui and L.J. Chang -- Detection of replication-competent lentiviral particles / H. Segall and R.E. Sutton -- Lymphocytes / D. Chinnasamy and F. Candotti -- Monocyte/macrophages and dendritic cells / H. Segall and R.E. Sutton -- Hematopoietic stem and progenitor cells / R.E. Donahue and I.S. Chen -- Mesenchymal stem cells / X.Y. Zhang, V.F. La Russa and J. Reiser -- Hippocampal neurons / J.L. Nadeau -- Dorsal root ganglia sensory neurons / J. Fleming, S.L. Ginn and I.E. Alexander -- Cardiomyocytes / D. Bonci, M.V. Latronico and G. Condorelli -- Airway epithelia / L.G. Johnson, S.H. Randell and J.C. Olsen -- Corneal cells / B. Appukuttan, T. McFarland and J.T. Stout -- Retinal tissue / T. McFarland, B. Appukuttan and J.T. Stout -- Hiv-2 vectors / S.K. Arya, J.E. Cho and S.H. Chang -- Siv vectors / A. Ruggieri, D. Negre and F.L. Cosset -- FIV vectors / N. Loewen [and others] -- Hybrid lentivirus vectors / V. Planelles -- Cells of respiratory epithelium / P.L. Sinn and P.B. McCray, Jr. -- Cells of the nervous system / C. Rosenblad and C. Lundberg.
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| Abstract
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Lentivirus vector-based technologies, through in vitro and in vivo gene transfer in eukaryotic animal cells, currently offer the most promising opportunities for curing genetic disorders, as well as cancer and infectious diseases. In Lentivirus Gene Engineering Protocols, Maurizio Federico has assembled a panel of outstanding experimenters to detail all the theoretical and practical aspects of lentivirus vector-based gene transfer. The authors demonstrate lentivirus vector production with detailed methods for recovering appropriate producer cells, for producing and titrating lentivirus-containing supernatants, and for detecting transduced cells. The applications of lentivirus vector engineering to different cell types include coverage of lymphocytes, dendritic cells, hematopoietic stem and progenitor cells, mesenchymal stem cells, hippocampal neurons, cardiomyocytes, as well as airway epithelia, corneal cells, and retinal pigment. The use of non-HIV-1-based lentivirus vectors is shown to be advantageous, particularly those obtained from the genomes of HIV-2, SIV, or FIV. Also advanced are applications in which genetic material is directly inoculated with lentivirus vectors in such experimental animal models as mouse, rat, and rabbit. Comprehensive and informative, Lentivirus Gene Engineering Protocols presents a broad spectrum of the latest methods of lentivirus vector technology, offering hematologists, neurologists, geneticists, and virologists the most promising and up-to-date tools available for gene therapy in such still fatal diseases as cancer, cystic fibrosis, and AIDS.
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Genetic engineering.
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Genetic vectors.
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Lentiviruses.
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Genetic engineering.
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Genetic vectors.
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Lentiviruses.
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SCIENCE-- Biotechnology.
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Lentivirus-- genetics.
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Genetische manipulatie.
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Lentivirussen.
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Wetenschappelijke technieken.
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Gene Targeting-- methods.
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Gene Transfer Techniques.
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Genetic Engineering-- methods.
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Genetic Vectors-- biosynthesis.
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| Dewey Classification
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660.6/5
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| LC Classification
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QH442.L455 2003
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| NLM classification
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QW 25L574 2003
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W1ME9616J v.229 2003
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| Added Entry
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Federico, Maurizio.
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