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" Plant secondary metabolism engineering : "
edited by Arthur Germano Fett-Neto.
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BL
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| Record Number
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1004899
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b759269
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| Title & Author
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Plant secondary metabolism engineering : : methods and applications /\ edited by Arthur Germano Fett-Neto.
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| Publication Statement
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New York :: Humana Press,, ©2010.
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| Series Statement
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Methods in molecular biology,; 643
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Springer protocols
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| Page. NO
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1 online resource (xii, 339 pages) :: illustrations (some color)
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| ISBN
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1607617234
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: 9781607617235
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1607617226
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9781607617228
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| Bibliographies/Indexes
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Includes bibliographical references and index.
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| Contents
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Plant secondary metabolism and challenges in modifying its operation : an overview / Naíla Cannes do Nascimento and Arthur Germano Fett-Neto -- Suppression subtractive hybridization as a tool to identify anthocyanin metabolism-related genes in apple skin / Yusuke Ban and Takaya Moriguchi -- Identification of regulatory protein genes involved in alkaloid biosynthesis using a transient RNAi system / Yasuyuki Yamada [and others] -- Site-directed mutagenesis and saturation mutagenesis for the functional study of transcription factors involved in plant secondary metabolite biosynthesis / Sitakanta Pattanaik [and others] -- Isolation of proteins binding to promoter elements of alkaloid metabolism-related genes using yeast one-hybrid / Débora Vom Endt and Giancarlo Pasquali -- Modulation of carotenoid accumulation in transgenic potato by inducing chromoplast formation with enhanced sink strength / Joyce Van Eck [and others] -- Over-expression of rate-limiting enzymes to improve alkaloid productivity / Tomoya Takemura [and others] -- Microbial expression of alkaloid biosynthetic enzymes for characterization of their properties / Hiromichi Minami, Nobuhiro Ikezawa, and Fumihiko Sato -- Producing a recombinant flavin-containing monooxygenase from Coffea arabica in Escherichia coli for screening of potential natural substrates / Igor Cesarino and Paulo Mazzafera -- Efficient production of active form recombinant cassava hydroxynitrile lyase using Escherichia coli in low-temperature culture / Hisashi Semba [and others] -- Introduction of the early pathway to taxol biosynthesis in yeast by means of biosynthetic gene cluster construction using SOE-PCR and homologous recombination / Pia Dahm and Stefan Jennewein -- Biocatalytic synthesis of tritium (³H)-labelled taxa-4(5), 11(12)-diene, the pathway committing precursor of the taxoid diterpenoids / Hans Schmeer and Stefan Jennewein -- USER cloning and USER fusion : the ideal cloning techniques for small and big laboratories / Hussam H. Nour-Eldin, Fernando Geu-Flores, and Barbara A. Halkier -- Enrichment of carotenoids in flaxseed by introducing a bacterial phytoene synthase gene / Masaki Fujisawa and Norihiko Misawa -- Metabolic engineering by plastid transformation as a strategy to modulate isoprenoid yield in plants / Tomohisa Hasunuma, Akihiko Kondo, and Chikahiro Miyake -- Engineering high yields of secondary metabolites in Rubia cell cultures through transformation with rol genes / Victor P. Bulgakov, yuri N. Shkryl, and Galina N. Veremeichik -- Flow cytometric methods to investigate culture heterogeneities for plant metabolic engineering / Vishal Gaurav, Martin E. Kolewe, and Susan C. Roberts -- Phenylpropanoid biosynthesis in leaves and glandular trichomes of basil (Ocimum basilicum L.) / Cícero Deschamps and James E. Simon -- Fusion with fluorescent proteins for subcellular localization of enzymes involved in plant alkaloid biosynthesis / Patrícia Duarte, Johan Memelink, and Mariana Sottomayor -- Immunohistochemical localisation of a putative flavonoid transporter in grape berries / Elisa Petrussa [and others] -- Electrogenic bromosulfalein transport in isolated membrane vesicles : implementation of both animal and plant preparations for the study of flavonoid transporters / Sabina Passamonti [and others].
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| Abstract
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Plants have evolved an amazing array of metabolic pathways leading to molecules capable of responding promptly and effectively to stress situations imposed by biotic and abiotic factors, some of which supply the ever-growing needs of humankind for natural chemicals, such as pharmaceuticals, nutraceuticals, agrochemicals, food and chemical additives, biofuels, and biomass. In Plant Secondary Metabolism Engineering: Methods and Applications, expert researchers provide detailed practical information on some of the most important methods employed in the engineering of plant secondary metabolism pathways and in the acquisition of essential knowledge in performing this activity, including the significant advances and emerging strategies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant Secondary Metabolism Engineering: Methods and Applications will aid scientists engaged in the challenging task of modifying some of the most intricate products of plant evolution and support their efforts directed toward the vital goal of sustainable natural chemicals.
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| Subject
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Biochemical engineering, Laboratory manuals.
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Biotransformation (Metabolism)-- Research, Laboratory manuals.
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Metabolism, Secondary-- Research, Laboratory manuals.
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Plants-- Effect of stress on-- Research, Laboratory manuals.
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Biochemical engineering.
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Biomedical Engineering.
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Health Biological Sciences.
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Biological Products-- biosynthesis
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Plants-- metabolism.
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Bioengineering.
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Genetic Techniques.
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| Dewey Classification
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660.63
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| LC Classification
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TP248.3.P53 2010
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| NLM classification
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QW 25
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W1ME9616J v.643 2010
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| Added Entry
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Fett-Neto, Arthur Germano,1967-
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