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" Protein kinase C protocols / "
edited by Alexandra C. Newton.
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BL
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| Record Number
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1011864
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b766234
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| Title & Author
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Protein kinase C protocols /\ edited by Alexandra C. Newton.
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| Publication Statement
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Totowa, N.J. :: Humana Press,, ©2003.
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| Series Statement
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Methods in molecular biology ;; v. 233
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| Page. NO
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1 online resource (xvi, 582 pages) :: illustrations
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| ISBN
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1280842717
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: 1588290689
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: 1592593976
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: 661084271X
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: 9781280842719
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: 9781588290687
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: 9781592593972
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: 9786610842711
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| Bibliographies/Indexes
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Includes bibliographical references and index.
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| Contents
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The ins and outs of protein kinase C / A.C. Newton -- Early studies of protein kinase C: A historical perspective / Y. Nishizuka and U. Kikkawa -- Expression and purification of protein kinase C from insect cells / H. Mukai and Y. Ono -- Expression and purification of protein kinase C[delta] from bacteria / M. Gschwendt and L. Stempka -- Complexities in protein kinase C activity assays: An introduction / J.J. Sando -- Enzyme assays for protein kinase C activity / J.J. Sando and J.K. Beals -- Subcellular translocation of protein kinase C / J.J. Sando, J.K. Beals and I.M. Hussaini -- Measuring the interaction of protein kinase C with membranes: An introduction / A.C. Newton -- Fluorescence imaging of protein kinase C translocation in living cells / N. Saito -- Measuring the binding of protein kinase C to sucrose-loaded vesicles / J. Giorgione and A.C. Newton -- Use of stopped-flow fluorescence spectroscopy to measure rapid membrane binding by protein kinase C / E.A. Nalefski and A.C. Newton -- [3H]Phorbol 12,13-dibutyrate binding assay for protein kinase C and related proteins / N.E. Lewin and P.M. Blumberg -- protein kinase C phosphorylation: An introduction / P.J. Parker -- Pulse-chase analysis of protein kinase C / M. Takahashi and Y. Ono -- PDK-1 and protein kinase C phosphorylation / A. Toker -- In vitro autophosphorylation of protein kinase C isozymes / A.M. Pepio and W.S. Sossin -- Tyrosine phosphorylation of protein kinase C / T. Yamamoto [and others] -- Methods to study dephosphorylation of protein kinase C in vivo / K. England and M. Rumsby -- Phosphopeptide-specific antibodies to protein kinase C / W.S. Sossin -- Identifying protein kinase C substrates: An introduction / A. Toker -- A chemical genetic approach for the identification of direct substrates of protein kinases / K. Shah and K.M. Shokat -- Studying the optimal peptide substrate motifs of protein kinase C using oriented peptide libraries / M.B. Yaffe -- Structural analysis of protein kinase C: An introduction / J.H. Hurley -- Bacterial expression and purification of C1 and C2 domains of protein kinase C isoforms / W. Cho [and others] -- Crystallization of the protein kinase C[delta] C1B domain / G. Zhang and J.H. Hurley -- Methods for detecting binding proteins: An introduction / G. Endemann and D. Mochly-Rosen -- Detection of protein kinase-binding partners by the yeast two-hybrid analysis / B.Y. Chang and C.A. Cartwright -- Glutathione S-transferase pull-down assay / D. Schechtman, D. Mochly-Rosen and D. Ron -- Overlay method for detecting protein-protein interactions / D. Schechtman [and others] -- An overlay assay for detecting protein kinase C-binding proteins and substrates / S. Jaken -- Functional proteomic analysis of the protein kinase C signaling system / J.M. Pass [and others] -- Pharmacological probes for protein kinase C: An introduction / M.G. Kazanietz -- Applications of inhibitors for protein kinase C and their isoforms / G.X. Shen [and others] -- Phorbol esters as probes for the study of protein kinase C function / M.G. Kazanietz and P.S. Lorenzo -- Irreversible inactivation of protein kinase C isozymes by thiol-reactive peptide substrate analogs / C.A. O'Brian [and others] -- Genetic approaches to studying protein kinase C: An introduction / R.O. Messing -- Animal models in the study of protein kinase C isozymes / D.S. Choi and R.O. Messing -- Genetic manipulation of protein kinase C in vivo / A. Toker -- Yeast as a host to screen for modulators and regulatory regions of mammalian protein kinase C isoforms / A.M. Parissenti and H. Riedel -- protein kinase C in disease: Cancer / A.P. Fields and W.C. Gustafson -- Characterization of the role of protein kinase C isozymes in colon carcinogenesis using transgenic mouse models / A.P. Fields, N.R. Murray and W.C. Gustafson -- Alcohol addiction / M.F. Olive and D. Ron.
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| Abstract
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Protein kinase C impacts on nearly every aspect of signal transduction, placing it at the forefront of research in how cells transduce information. In Protein Kinase C Protocols, leading investigators from academia and industry present a comprehensive collection of classical and emerging laboratory techniques for the study of this influential protein, all optimized for successful and reproducible results. Described in step-by-step detail, these methods can be easily used to explore the structure, function, regulation, subcellular localization, and macromolecular interactions of protein kinase C. Included in this collection are easy-to-follow techniques to purify protein kinase C, to measure its activity and its membrane interaction in vivo and in vitro, and to identify substrates. There are also methodologies and techniques for measuring the phosphorylation state of protein kinase C, for expressing and analyzing the membrane-targeting domains of protein kinase C, and for detecting binding proteins. Additional chapters address pharmacological probes, genetic approaches, and protein kinase C in disease. Each protocol is introduced in the context of protein kinase C function and regulation and contains many notes on how best to deal with the problems that may occur. Comprehensive and authoritative, Protein Kinase C Protocols offers a timely compilation of biophysical, biochemical, cell biological, and molecular biological approaches that will ensure successful results in every laboratory working in this rapidly advancing field.
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| Subject
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Protein kinase C, Laboratory manuals.
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Protéine kinase C, Manuels de laboratoire.
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| Subject
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Protein kinase C.
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| Subject
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SCIENCE-- Life Sciences-- Biochemistry.
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| Subject
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Protein Kinase C.
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Signal Transduction.
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| Dewey Classification
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572/.792
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| LC Classification
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QP606.P76P7353 2003
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| NLM classification
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QU 141P9661 2003
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W1ME9616J v.233 2003
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| Added Entry
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Newton, Alexandra C.
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