| Document Type
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BL
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| Record Number
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1023357
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| Doc. No
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b777727
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| Title & Author
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Immunochemical protocols /\ edited by Margaret M. Manson.
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| Publication Statement
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Totowa, N.J. :: Humana Press,, ©1992.
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| Series Statement
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Methods in molecular biology ;; v. 10
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| Page. NO
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xiii, 480 pages :: illustrations ;; 24 cm
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| ISBN
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0896032043
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: 0896032701
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: 1592594972
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: 9780896032040
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: 9780896032705
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: 9781592594979
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| Bibliographies/Indexes
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Includes bibliographical references and index.
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| Contents
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Production of polyclonal antisera -- Efficient elution of purified proteins from polyvinylidene difluoride membranes (immobilon) after transfer from SDS-PAGE and their use as immunogens -- Raising polyclonal antibodies using nitrocellulose-bound antigen -- Synthesis of peptides for use as immunogens -- Production and characterization of antibodies against synthetic peptides -- Preparation and testing of monoclonal antibodies to recombinant proteins -- Screening of monoclonal antibodies using antigens labeled with acetylcholinesterase -- Purification of immunoglobulin G (IgG) -- Epitope mapping -- Enzyme-antienzyme method for immunohistochemistry -- Double label immunohistochemistry on tissue sections using alkaline phosphatase and peroxidase conjugates -- Immunohistochemical detection of bromodeoxyuriding-labeled nuclei for in vivo cell kinetic studies -- Avidin-biotin technology: preparation of biotinylated probes -- Avidin-biotin technology: preparation of avidin conjugates -- Immunochemical applications of avidin-biotin technology -- Preparation of gold probes -- Immunogold probes for light microscopy -- Immunogold probes in electron microscopy -- Electron microscopic silver enhancement for double labeling with antibodies raised in the same species -- Quantitative and qualitative immunoelectrophoresis -- Rocket immunoelectrophoresis techniques or electroimmunodiffusion -- Crossed immunoelectrophoresis -- Crossed immunoaffinoelectrophoresis -- Immunodetection of proteins by western blotting -- Erasable western blots -- Colloidal gold staining and immunoprobing on the same western blot -- Colloidal gold staining and immunodetection in 2-D protein mapping -- Fluorescent protein staining on nitrocellulose with subsequent immunodetection of antigen -- Competitive ELISA -- Twin-site ELISAs for fos and myc oncoproteins using the AMPAK system -- Preparation of cytotoxic antibody-toxin conjugates -- Immunoaffinity purification and quantification of antibody-toxin conjugates -- An immuno-slot-blot assay for detection and detection and quantitation of alkyldeoxyguanosines in DNA -- Production of monoclonal antibodies for the detection of chemically modified DNA -- Sensitive competitive enzyme-linked immunoassay for quantitation of modified bases in DNA -- An immunochemical assay for detecting transition of B-DNA to Z-DNA -- Cell sorting using immunomagnetic beads -- Cell preparation for flow cytometry -- Preparation of rat lung cells for flow cytometry -- The isolation of rat hepatocytes for flow cytometry -- Flow cytometric analysis of cells using an antibody to a surface antigen -- Multiple immunofluorescence analysis of cells using flow cytometry -- Cell kinetic studies using a monoclonal antibody to bromodeoxyuridine -- Production and use on nonradioactive hybridization probes -- Cellular human and viral DNA detection by nonisotopic in situ hybridization -- Chromosomal mapping of genes by nonisotopic in situ hybridization -- Nonisotopic in situ hybridization -- Biotinylated probes in colony hybridization -- Screening of agt11 cDNA libraries using monoclonal antibodies -- Expression of foreign genes in mammalian cells using an antibody fusion system.
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| Subject
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Immunochemistry-- Methodology.
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Antibodies-- isolation purification.
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Immunochemistry-- methods.
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Immunochimie-- Méthodologie.
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Aufsatzsammlung
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Immunchemie
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Immunochemistry-- Methodology.
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Immunochimie-- Technique.
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Immunochimie.
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Methode
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Techniques immunologiques.
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Immunochemie.
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| Subject
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Wetenschappelijke technieken.
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| Dewey Classification
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616.07/56
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| LC Classification
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QR183.6.I43 1992
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| NLM classification
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W1ME9616J v.10 1992
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QW 25I334 1992
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44.45bcl
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CHE 810fstub
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CHE 880fstub
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MED 460fstub
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WC 4150rvk
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YV 4500rvk
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| Added Entry
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Manson, Margaret M.
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