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" Transgenic maize : "
edited by M. Paul Scott.
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BL
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| Record Number
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1024625
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b778995
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| Title & Author
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Transgenic maize : : methods and protocols /\ edited by M. Paul Scott.
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| Publication Statement
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New York, NY :: Humana,, ©2009.
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| Series Statement
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Methods in molecular biology,; 526
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| Page. NO
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1 online resource (ix, 180 pages, [8] pages of color plates) :: illustrations
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| ISBN
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159745494X
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: 1934115495
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: 9781597454940
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: 9781934115497
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| Bibliographies/Indexes
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Includes bibliographical references and index.
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| Contents
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Design of gene constructs for transgenic maize / D. Liu -- Transient expression of GFP in immature seed tissues / C.T. Shepherd -- Biolistic gun-mediated maize genetic transformation / K. Wang and B. Frame -- Agrobacterium-mediated maize transformation: Immature embryos versus callus / V. Sidorov and D. Duncan -- Whiskers-mediated maize transformation / J.F. Petolino and N.L. Arnold -- Methods for generation and analysis of fluorescent protein-tagged maize lines / A. Mohanty [and others] -- Use of transgene-induced RNAi to regulate endogenous gene expression / K. McGinnis -- Plasmid rescue: Recovery of flanking genomic sequences from transgenic transposon insertion sites / G.-L. Nan and V. Walbot -- Nonradioactive genomic DNA blots for detection of low abundant sequences in transgenic maize / G.-L. Nan and V. Walbot -- Tissue-print immunodetection of transgene products in endosperm for high-throughput screening of seeds / M.P. Scott -- Determination of transgene copy number by real-time quantitative PCR / C.T. Shepherd, A.N. Moran Lauter and M.P. Scott -- Herbicide resistance screening assay / J.M. Peterson -- Characterizing transgene inheritance / E.H. Bicar -- Backcross breeding / K.E. Vogel.
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| Abstract
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As increasing global population and continuing economic development ensure the need for further production and cultivation of maize, the necessity of the application of transgenic technology to this model species and crop plant grows steadily. In Transgenic Maize: Methods and Protocols, experts in the various disciplines of the field contribute readily reproducible protocols covering such topics as transformation methods, transgenic maize in research, the analysis of transgenic plants, and breeding with transgenes. Written in the highly successful Methods in Molecular Biology series format, the chapters present brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Cutting-edge and easy to use, Transgenic Maize: Methods and Protocols will greatly aid researchers wishing to use these procedures in their own laboratories, and will provide readers with a better understanding of the experiments being performed by cooperators or fee-for-service labs.
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| Subject
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Corn-- Genetic engineering.
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Transgenic plants.
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Corn-- Genetic engineering.
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Transgenic plants.
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Corn-- Genetic engineering.
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Plants, Genetically Modified.
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Transgenic plants.
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Zea mays-- genetics.
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Zea mays-- genetics.
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Plants, Genetically Modified.
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| Dewey Classification
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633.15233
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| LC Classification
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SB191.M2T725 2009
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| NLM classification
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SB 191.M2T772 2009
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W1ME9616J v.526 2009
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S513. 03clc
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| Added Entry
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Scott, M. Paul.
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