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" Chromatin protocols / "
edited by Srikumar P. Chellappan.
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BL
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| Record Number
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1024649
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b779019
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| Title & Author
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Chromatin protocols /\ edited by Srikumar P. Chellappan.
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| Edition Statement
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2nd ed.
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| Publication Statement
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New York :: Humana Press/Springer,, ©2009.
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| Series Statement
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Methods in molecular biology,; v. 523
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Springer protocols
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1 online resource (xiii, 416 pages) :: illustrations
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| ISBN
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1597451908
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: 9781597451901
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1588298736
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9781588298737
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| Bibliographies/Indexes
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Includes bibliographical references and index.
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| Contents
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Preparation of chromatin assembly extracts from preblastoderm Drosophila embryos / Edgar Bonte and Peter B. Becker -- Analysis of reconstituted chromatin using a solid-phase approach / Raphael Sandaltzopoulos and Peter B. Becker -- In vivo chromatin decondensation assays : molecular genetic analysis of chromatin unfolding characteristics of selected proteins / Sherry L. Winter, Philip Wong, and Mark G. Alexandrow -- DNA methyltransferase probing of chromatin structure within populations and on single molecules / Carolina Pardo [and others] -- Visualization of the expression of HMGN nucleosomal binding proteins in the developing mouse embryo and in adult mouse tissues / Takashi Furusawa and Michael Bustin -- Drug-induced premature chromosome condensation (PCC) protocols : cytogenetic approaches in mitotic chromosome and interphase chromatin / Eisuke Gotoh -- Analysis of DNA topology in yeast chromatin / Randall H. Morse -- Preparation and analysis of uniquely positioned mononucleosomes / Daria A. Gaykalova [and others] -- Monitoring DNA breaks in optically highlighted chromatin in living cells by laser scanning confocal microscopy / Michael J. Kruhlak, Arkady Celeste, and André Nussenzweig -- Methods to study transcription-coupled repair in chromatin / Hélène Gaillard, Ralf Erik Wellinger, and Andrés Aguilera -- Cytometric analysis of DNA damage : phosphorylation of histone H2AX as a marker of DNA double-strand breaks (DSBs) / Toshiki Tanaka [and others] -- Detection of DNA damage induced by topoisomerase II inhibitors, gamma radiation, and crosslinking agents using the comet assay / Lori A. Hazlehurst -- Analysis of genomic aberrations using comparative genomic hybridization of metaphase chromosomes / Melanie Carless -- In vitro replication assay with mammalian cell extracts / Wasia Rizwani and Srikumar P. Chellappan -- Investigation of genomic methylation status using methylation-specific and bisulfite sequencing polymerase chain reaction / Melanie Carless -- Biochemical analysis of arginine methylation in transcription / Marc Tini, Hina Naeem, and Joseph Torchia -- In vitro histone demethylase assays / Kenji Kokura and Jia Fang -- Histone chaperone as coactivator of chromatin transcription : role of acetylation / Shrikanth S. Gadad [and others] -- Histone deacetylase activity assay / Zhigang Yuan [and others] -- In vitro and in vivo assays for studying histone ubiquitination and deubiquitination / Ling Zhai, Heui-Yun Joo, and Hengbin Wang -- Detection of histone H3 phosphorylation in cultured cells and tissue sections by immunostaining / Jaya Padmanabhan -- Chromatin immunoprecipitation assays : analyzing transcription factor binding and histone modifications in vivo / Smitha Pillai, Piyali Dasgupta, and Srikumar P. Chellappan -- ChIP on Chip assays : genome-wide analysis of transcription factor binding and histone modifications / Smitha Pillai and Srikumar P. Chellappan -- ChIP-on-Chip analysis methods for Affymetrix tiling arrays / Sean J. Yoder and Steven A. Enkemann -- Non-radioactive assay methods for the assessment of telomerase activity and telomere length / Partha P. Banerjee and Shankar Jagadeesh -- Detecting ATM-dependent chromatin modification in DNA damage and heat shock response / Sandeep Misri, Shruti Pandita, and Tej K. Pandita.
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| Abstract
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This volume encompasses a wide array of techniques spanning from isolation of nucleosomes, assembly of nucleosomes and study of the basic chromatin structure to detailed analysis of histone modifications and chromatin function.
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Chromatin-- Structure.
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Eukaryotic cells.
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Eukaryotic cells.
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Chromatin-- ultrastructure.
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Eukaryotic Cells.
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| Dewey Classification
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572.87
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| LC Classification
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QH599
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| NLM classification
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QU 25C5565 2009
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W1ME9616J v.523 2009
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| Added Entry
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Chellappan, Srikumar P.
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