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" The urinary proteome : "
edited by Alex J. Rai.
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BL
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| Record Number
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1025306
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b779676
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| Title & Author
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The urinary proteome : : methods and protocols /\ edited by Alex J. Rai.
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| Publication Statement
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[Totowa, N.J.] :: Humana Press,, ©2010.
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| Series Statement
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Methods in molecular biology,; 641
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Springer protocols
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| Page. NO
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1 online resource (xi, 361 pages) :: illustrations (some color)
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| ISBN
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1607617110
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: 9781607617112
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1607617102
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9781607617105
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| Bibliographies/Indexes
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Includes bibliographical references and index.
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| Contents
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Introduction to urinalysis: historical perspectives and clinical application / Germán Echeverry, Glen L. Hortin and Alex J. Rai -- A primer on clinical applications and assays using urine: focus on analysis of plasma cell dyscrasias using automated electrophoresis and immunofixation / Brianne Olivieri and Alex J. Rai -- Application of free flow electrophoresis to the analysis of the urine proteome / Aude L. Foucher, David R. Craft and Crag A. Gelfand -- Standardized preprocessing of urine for proteome analysis / Georg Martin Fiedler [and others] -- Different sample preparation and detection methods for normal and lung cancer urinary proteome analysis / Supachok Sinchaikul [and others] -- Isolation and purification of exosomes in urine / Patricia A. Gonzales [and others] -- Bioinformatics of the urinary proteome / Laurence D. Parnell and Christine M.E. Schueller -- PROTIS: use of combined biomarkers for providing diagnostic information on disease states / Walter Hofmann [and others] -- Statistical contributions to proteomic research / Jeffrey S. Morris [and others] -- A review of experimental design best practices for proteomics based biomarker discovery: focus on SELDI-TOF / Rebecca E. Caffrey -- Urine proteomic profiling for biomarkers of acute renal transplant rejection / Shu-Ling Liang and William Clarke -- Surface plasmon resonance biosensorics in urine proteomics / Peter B. Luppa, Jochen Metzger, and Heike Schneider -- Urinary proteins for the diagnosis of obstructive sleep apnea syndrome / Ayelet Snow [and others] -- Immune response biomarker profiling application on ProtoArray protein microarrays / Barry Schweitzer [and others] -- Design and validation of an immunoaffinity LC-MS/MS assay for the quantification of a collagen type II neoepitope peptide in human urine: application as a biomarker of osteoarthritis / Olga Nemirovskiy, Wenlin Wendy Li, and Gabriella Szekely-Klepser -- Cell-specific biomarkers in renal medicine and research / Martin Shaw -- Proteomic assays for the detection of urothelial cancer / Kris E. Gaston and H. Barton Grossman -- Urine proteomic analysis: use of two-dimensional gel electrophoresis, isotope coded affinity tags, and capillary electrophoresis / Kimia Sobhani -- Proteomic analysis of pancreatic secretory trypsin inhibitor/tumor-associated trypsin inhibitor from urine of patients with pancreatitis or prostate cancer / Leena Valmu, Suvi Ravela, and Ulf-Håkan Stenman.
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| Abstract
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Urine is one of the most easily accessible biological samples, and it provides a treasure trove of molecules essential for clinical diagnostics. In The Urinary Proteome: Methods and Protocols, expert researchers review briefly the classical urine tests that are performed in the clinical laboratory and then delve into the state of the art methods for proteomic analysis using urine specimens. Featuring the most recent advances in sample preparation, data analysis, and methods and applications, the book contains a multitude of detailed methods, including procedural details for the identification and characterization of urine biomarkers, which hold great potential for the diagnosis and treatment of many different disease conditions. Written in the highly successful Methods in Molecular Biology series format, chapters present brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes highlighting tips on troubleshooting and avoiding known pitfalls. Authoritative and systematic, The Urinary Proteome: Methods and Protocols is an ideal guide for scientific researchers, clinical laboratorians, clinical and translational scientists, and others interested in the vital study of proteomics and biomarker discovery.
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| Subject
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Proteomics-- Methodology.
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Urine-- Analysis.
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Urine-- Analysis.
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Proteomics-- methods.
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Urinalysis-- methods.
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Biomarkers.
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| Subject
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Urine-- chemistry.
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| Dewey Classification
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572.60724
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| LC Classification
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QP551.U75 2010
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| NLM classification
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QY 185
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| Added Entry
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Rai, Alex J.
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