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" Posttranslational modifications of proteins : "
edited by Christoph Kannicht.
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BL
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| Record Number
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1034423
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b788793
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| Title & Author
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Posttranslational modifications of proteins : : tools for functional proteomics /\ edited by Christoph Kannicht.
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| Publication Statement
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Totowa, NJ :: Humana Press,, ©2002.
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| Series Statement
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Methods in molecular biology ;; v. 194
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| Page. NO
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1 online resource (xi, 322 pages) :: illustrations
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| ISBN
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0585403163
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: 0896036782
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: 1280821434
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: 1592591817
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: 6610821437
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: 9780585403168
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: 9780896036789
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: 9781280821431
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: 9781592591817
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: 9786610821433
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| Bibliographies/Indexes
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Includes bibliographical references and index.
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| Contents
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Assignment of disulfide bonds in proteins by chemical cleavage and peptide mapping by mass spectrometry / Jiang Wu -- Carbohydrate composition analysis of glycoproteins using highly sensitive fluorescence detection methods / George N. Saddic -- Enzymatical hydrolysis of N-glycans from glycoproteins and fluorescent labeling by 2-aminobenzamide (2-AB) / Rolf Nuck -- Separation of N-glycans by HPLC / Martin Gohlke -- Enzymatic sequence analysis of N-glycans / Christoph Kannicht -- Immunological detection of O-GlcNAc / Monika Rex-Mathes -- Analysis of O-glycosylation / Juan J. Calvete -- Characterization of site-specific glycosylation / Katalin F. Medzihradszky -- Monitoring glycosylation of therapeutic glycoproteins for consistency using highly fluorescent anthranilic acid / Shirish T. Dhume -- Metabolic labelling and structural analysis of glycosylphosphatidylinositols from parasitic protozoa / Peter Gerold -- Analysis of S-acylation of proteins / Michael Veit -- Immunoblotting methods for the study of protein ubiquitination / Edward G. Mimnaugh -- Analysis of methylation and acetylation in E. coli ribosomal proteins / Randy J. Arnold -- Identification of in vivo protein phosphorylation sites with mass spectrometry / Jun Qin -- Analysis of tyrosine-O-sulfation / Jens R. Bundgaard -- a-amidated peptides: approaches for analysis / Gregory P. Mueller -- y-glutamate and B-hydroxyaspartate in proteins / Francis J. Castellino -- Detection of isoAspartate residues as a posttranslational modification of proteins and peptides / Verne Schirch -- Lysine hydroxylation and crosslinking of collagen / Mitsuo Yamauchi -- Heterologous expression in endocrine cells for analysis of posttranslational modifications / Jens R. Bundgaard -- 2D-electrophoresis: detection of glycosylation and influence on spot pattern / Klemens Löster.
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| Abstract
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The majority of all proteins undergo posttranslational modifications that significantly alter their physical and chemical properties, including their folding and conformation distribution, their stability, and, consequently, their activity and function. In Posttranslational Modifications of Proteins: Tools for Functional Proteomics, Christoph Kannicht and a panel of highly experienced researchers describe readily reproducible methods for detecting and analyzing the most important of these modifications, particularly with regard to protein function, proteome research, and the characterization of pharmaceutical proteins. Among the methods presented are those for analyzing the assignment of disulfide bond sites in proteins, protein N-glycosylation and protein O-glycosylation, and oligosaccharides present at specific single glycosylation sites in a protein. Additional powerful techniques facilitate the analysis of glycosylphosphatidylinositols, lipid modifications, protein phosphorylation and sulfation, protein methylation and acetylation, a-amidation, g-glutamate, isoaspartate, and lysine hydroxylation. Comprehensive and state-of-the-art, Posttranslational Modifications of Proteins: Tools for Functional Proteomics serves as a highly practical guide for all investigators of protein structure-function relationships not only in chemical and pharmaceutical research, but also throughout the rapidly growing field of functional proteomics.
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| Subject
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Post-translational modification, Laboratory manuals.
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| Subject
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Protein Processing, Post-Translational.
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Post-translational modification.
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SCIENCE-- Life Sciences-- Biochemistry.
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| Dewey Classification
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572/.6
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| LC Classification
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QH450.6.P685 2002
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| NLM classification
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QU 25P858 2002
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W1ME9616J v.194 2002
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| Added Entry
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Kannicht, Christoph.
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