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" Antibiotic resistance protocols / "
edited by Stephen H. Gillespie, Timothy D. McHugh.
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BL
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| Record Number
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1034845
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b789215
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| Title & Author
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Antibiotic resistance protocols /\ edited by Stephen H. Gillespie, Timothy D. McHugh.
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| Edition Statement
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2nd ed.
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| Publication Statement
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New York :: Humana Press,, 2010.
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| Series Statement
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Methods in molecular biology,; 642
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Springer protocols
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1 online resource (x, 227 pages) :: illustrations
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| ISBN
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1603272798
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: 9781603272797
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160327278X
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9781603272780
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| Notes
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Revised edition of: Antibiotic resistance. c2001.
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| Bibliographies/Indexes
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Includes bibliographical references and index.
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| Contents
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Application of continuous culture for measuring the effect of environmental stress on mutation frequency in Mycobacterium tuberculosis / J. Bacon, K.A. Hatch, and J. Allnutt -- Identification of efflux-mediated multi-drug resistance in bacterial clinical isolates by two simple methods / M. Martins [and others] -- Evaluation of efflux activity of bacteria by a semi-automated fluorometric system / M. Viveiros [and others] -- Measuring the activity of active efflux in gram-negative bacteria / M.A. Webber and N.G. Coldham -- Rapid characterization of beta-lactamases by multiplex PCR / N. Woodford -- Rapid methods for testing inhibitors of mycobacterial growth / D. Evangelopoulos and S. Bhakta -- Use of biofilm model systems to study antimicrobial susceptibility / J. Pratten and D. Ready -- Rapid analysis of resistant mutant genotypes using pyrosequencing / C. Arnold.
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The RM test for determining methicillin-resistant Staphylococcus aureus lineages / J.A. Lindsay and J.M. Sung -- Unravelling the ecology of antibiotic resistant bacteria in the nasopharynx / B.M. Charalambous and N.M. Oriyo -- Methods to determine antibiotic resistance gene silencing / V.I. Enne and P.M. Bennett -- Studying gene induction of glycopeptide resistance using gene swapping / H.J. Hong -- Real-time PCR methods to study expression of genes related to hypermutability / D.M. O'Sullivan -- Use of DNA arrays to study transcriptional responses to antimycobacterial compounds / S.J. Waddell and P.D. Butcher -- Assays of sensitivity of antibiotic-resistant bacteria to hydrogen peroxide and measurement of catalase activity / M. Macvanin and D. Hughes -- Measurements of heme levels and respiration rate in Salmonella enterica serovar typhimurium / D. Hughes and M. Macvanin -- Methods to determine fitness in bacteria / C.F. Pope, T.D. McHugh, and S.H. Gillespie.
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| Abstract
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Since the publication of the popular first edition, genomic methods have become more accessible, allowing antibiotic researchers to probe not only the sequence of antibiotic resistance determinants but the mechanism whereby they are expressed and regulated. That, in concert with array technology and an understanding of the importance of biofilms, has greatly expanded antibiotic resistance knowledge. In order to reflect the growing field, Antibiotic Resistance Protocols, Second Edition fully updates and builds upon its first edition with contributions from leading researchers. Beginning with chapters on epidemiology and population genetics, the book continues with sections covering genomics and gene expressions, fitness mutation and physiology, and the detection of resistance. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Antibiotic Resistance Protocols, Second Edition brings together examples of a diverse range of modern techniques applied in antibiotic research in order to best aid scientists in planning their own future research.
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Antibiotics, Laboratory manuals.
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Drug resistance in microorganisms, Laboratory manuals.
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Anti-Bacterial Agents-- therapeutic use.
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Anti-Infective Agents-- therapeutic use.
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Clinical Protocols.
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Drug Resistance, Microbial-- physiology.
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Antibiotics.
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Drug resistance in microorganisms.
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| Dewey Classification
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616.9041078
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| LC Classification
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QH506.M45 v.642 2010
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| NLM classification
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QW 45
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W1ME9616J v.642 2010
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| Added Entry
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Gillespie, S. H.
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McHugh, Timothy D., (Timothy Daniel)
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