| Document Type
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BL
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| Record Number
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1035650
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b790020
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| Title & Author
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Methods in cell biology.\ edited by David M. Prescott.
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| Publication Statement
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New York :: Academic Press,, 1978.
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| Series Statement
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Methods in cell biology
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| Page. NO
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1 online resource (xix, 539 pages) :: illustrations.
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| ISBN
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0080859178
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: 0125641206
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: 1282167677
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: 661216767X
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: 9780080859170
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: 9780125641203
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: 9781282167674
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: 9786612167676
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9780080859170
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| Bibliographies/Indexes
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Includes bibliographical references and index.
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| Contents
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Front Cover; Methods in Cell Biology, Volume XX; Copyright Page; Contents; List of Contributors; Preface; Chapter 1. Measuring Spontaneous Mutation Rates in Yeast; I. Introduction; II. Measuring the Spontaneous Mutation Rate in Mitotic Cells by Limiting a Required Nutrient; III. Measuring the Spontaneous Mutation Rate in Mitotic Cells Grown in Complete Medium; IV. Measuring the Spontaneous Mutation Rate during Meiosis; V. Conclusion; Appendix: Biometrical Aspects of Measuring Mutation Rates; References; Chapter 2. Manganese Mutagenesis in Yeast; I. Introduction
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Chapter 6. Method for Yeast Protein Synthesis in a Cell-Free SysternI. Introduction; II. Early Yeast Cell-Free Systems; III. The S-30 Extract; IV. Assay of Protein Synthesis; V. Prospects; References; Chapeter 7. Preparation of Protoplasts of Schizosaccharomyces pombe; I. Introduction; II. Preparation of Protoplasts; III. Concluding Remarks; References; Chapter 8. Bulk Isolation of Yeast Mitochondria; I. Introduction; II. Materials and Methods; III.Breakage of Yeast Cells; IV.Isolation of Mitochondria; V.Properties of Isolated Mitochondria; VI.Discussion; References
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Chapter 9. Isolation, Regeneration, and Fusion of Protoplasts of PhycomycesI. Introduction; II. Material; III. Preparation of Cells; IV.Protoplast Isolation; V.Reversion of Protoplasts; VI.Protoplast Fusion; VII.Discussion; References; Chapter 10.The Neurospora Plasma Membrane: A New Experimental System for Investigating Eukaryote Surface Membrane Structure and Function; I. Introduction; II.Isolation of Neurospora Plasma Membranes; III.Properties of Isolated Neurospora Plasma Membranes; IV.Conclusion; References; Chapter 11. Staining Fungal Nuclei with Mithramycin; I.Introduction
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I. Preparation of RibosomesII. Preparation of Ribosomal Proteins; III. Analysis of Ribosomal Proteins; IV. Conclusion; References; Note Added in Proof; Chapter 5. Isolation, Characterization, and Translation of mRNA from Yeast; I. Media, Cells, and Spheroplasts; II. Isolation of Polysomes, Single Ribosomes, and Ribosomal Subunits; III. Detection of mRNA in Polysomes and Subunits; IV. Preparation of RNA; V. Analysis of RNA on Polyacrylamide Gels; VI. Isolation of Poly-A-Containing RNA; VII. Analysis of 5'-Termini of mRNA; VIII. Translation of Yeast mRNA; References
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II. Induction of Mitochondrial MutationsIII. Induction of Nuclear Mutations; IV. The Uptake of Manganese and Its General Effects on Yeast Cells; V. Other Mutagens Inducing Mutations Nonlethal for rho Factor; References; Chapter 3. Yeast Cell Selection and Synchrony: Density Gradients and Mating Factor Block; I. Introduction; II. lsopycnic Density Gradients; III. Isolation of Cells Labeled at Specific Periods of the Cell Cycle; IV. a- and a-Factor Selection and Synchronization of Haploid Yeast Cells; V. Discussion; References; Chapter 4. The Ribosomal Proteins of Saccharomyces cerevisiae
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| Abstract
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METHODS IN CELL BIOLOGY,VOLUME 20.
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| Subject
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Cytology-- Technique.
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| Subject
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Cytology-- Technique.
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| Dewey Classification
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571.6072
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| LC Classification
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QH585.M48 1978eb
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| Added Entry
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Prescott, David M.,1926-2011.
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