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" RT-PCR protocols. "
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BL
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| Record Number
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1040314
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b794684
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| Title & Author
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RT-PCR protocols.
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| Edition Statement
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2nd ed. // edited by Nicola King.
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| Publication Statement
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New York :: Humana,, ©2010.
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| Series Statement
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Methods in molecular biology,; 630
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Springer protocols
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| Page. NO
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1 online resource (xi, 341 pages) :: illustrations
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| ISBN
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1607616289
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: 1607616297
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: 9781607616283
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: 9781607616290
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| Bibliographies/Indexes
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Includes bibliographical references and index.
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| Contents
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Single cell RT-PCR on mouse embryos : a general approach for developmental biology / Wolfgang Mann and Thomas Haaf -- Poly(A) cDNA real-time PCR for indicator gene measurement in cancer / Melissa Oliveira-Cunha, Ajith K. Siriwardena, and Richard J. Byers -- Transcriptome profiling of host-microbe interactions by differential display RT-PCR / Leong Wai Fook and Vincent T.K. Chow -- Quantitative RT-PCR methods for mature microRNA expression analysis / Stephanie D. Fiedler, Martha Z. Carletti, and Lane K. Christenson -- Detection of influenza A virus neuraminidase and PB2 gene segments by one step reverse transcription polymerase chain reaction / Alejandra Castillo Alvarez [and others] -- Detection and identification of CD46 splicing isoforms by nested RT-PCR / Anita Szalmás [and others] -- Simultaneous detection of bluetongue virus RNA, internal control GAPDH mRNA, and external control synthetic RNA by multiplex real-time PCR / Frank Vandenbussche, Elise Vandemeulebroucke, and Kris De Clercq -- Detection of West Nile viral RNA from field-collected mosquitoes in tropical regions by conventional and real-time RT-PCR / Ana Silvia González-Reiche [and others] -- Detection of antisense RNA transcripts by strand-specific RT-PCR / Eric C.H. Ho, Michael E. Donaldson, and Barry J. Saville -- RT-PCR amplification and cloning of large viral sequences / Xiaofeng Fan and Adrian M. Di Bisceglie -- One-step RT-LATE-PCR for mRNA and viral RNA detection and quantification / Cristina Hartshorn and Lawrence J. Wangh -- Changes in gene expression of caveolin-1 after inflammatory pain using quantitative real-time PCR / Fiza Rashid-Doubell -- Real-time quantitative reverse transcriptase polymerase chain reaction / Hongxin Fan and Ryan S. Robetorye -- The use of comparative quantitative RT-PCR to investigate the effect of cysteine incubation on GPx1 expression in freshly isolated cardiomyocytes / Nicola King -- The renaissance of competitive PCR as an accurate tool for precise nucleic acid quantification / Lorena Zentilin and Mauro Giacca -- Skeletal muscle RNA extraction in preparation for RT-PCR / Janelle P. Mollica -- Reverse transcription of the ribonucleic acid : the first step in RT-PCR assay / Fadia Haddad and Kenneth M. Baldwin -- Primer design for RT-PCR / Kelvin Li and Anushka Brownley -- Hot start PCR / Natasha Paul, Jonathan Shum, and Tony Le -- Real-time RT-PCR for automated detection of HIV-1 RNA during blood donor screening / Jens Müller.
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| Abstract
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Once a tedious, highly skilled operation, reverse-transcription polymerase chain reaction (RT-PCR) has become a routine and invaluable technique used in most laboratories. In RT-PCR Protocols, Second Edition, expert researchers fully update the technologies presented in the popular previous edition, such as competitive RT-PCR, nested RT-PCR, RT-PCR from single cells, and RT-PCR for cloning. In addition, newer technologies are also explored, including multiplex RT-PCR, RT-LATE-PCR, and the greatly advanced field of real-time quantitative RT-PCR, while recent advances in creating the optimum RT-PCR reaction, e.g. RNA extraction, primer design, and reverse transcription, end the book with their indispensable input. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes sections, highlighting tips on troubleshooting and avoiding known pitfalls. User friendly and up-to-date, RT-PCR Protocols, Second Edition acts as a handy companion to scientists from numerous diverse backgrounds who wish to explore further the marvels of gene expression.
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| Subject
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Polymerase chain reaction, Laboratory manuals.
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| Subject
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Polymerase chain reaction.
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| Subject
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Reverse Transcriptase Polymerase Chain Reaction.
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| Dewey Classification
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572.43
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| LC Classification
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QP606.D46R8 2010
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| NLM classification
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QU 450
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W1ME9616J v.630 2010
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WC 4460rvk
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| Added Entry
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King, Nicola,Dr.
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