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BL
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| Record Number
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1040956
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b795326
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| Title & Author
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Protocols for nucleic acid analysis by nonradioactive probes.
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| Edition Statement
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2nd ed. // edited by Elena Hilario, John Mackay.
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| Publication Statement
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Totowa, N.J. :: Humana Press,, ©2007.
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| Series Statement
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Methods in molecular biology,; 353
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| Page. NO
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1 online resource (x, 321) :: illustrations
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| ISBN
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1280832703
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: 1588294307
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: 1597452297
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: 6610832706
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: 9781280832703
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: 9781588294302
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: 9781597452298
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: 9786610832705
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| Bibliographies/Indexes
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Includes bibliographical references and index.
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| Contents
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Genomic DNA isolation from different biological materials / Duckchul Park -- Extraction of plant RNA / Elspeth MacRae -- Overview of hybridization and detection techniques / Elena Hilario -- Checkerboard DNA-DNA hybridization technology : using digoxigenin detection / Lisa S. Gellen, Glenn M. Wall-Manning, and Chris H. Sissons -- Nonradioactive northern and southern analyses from plant samples / Christoph Peterhaensel, Dagmar Weier, and Thomas Lahaye -- Screening a BAC library with nonradioactive overlapping oligonucleotide (overgo) probes / Elena Hilario, Tiffany F. Bennell, and Erik Rikkerink -- Direct in-gel hybridization of DNA with digoxigenin-labeled probes / Saeed A. Khan and Mohamed S. Nawaz.
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In situ hybridization of termite microbes / Shigeharu Moriya [and others] -- RNA electrophoretic mobility shift assay using a fluorescent DNA sequencer / Yukinori Eguchi -- Comparative quantitation of mRNA expression in the central nervous system using fluorescence in situ hybridization / Darren J. Day, Eli M. Mrkusich, and John H. Miller -- Visualization of gene expression by fluorescent multiplex reverse transcriptase-PCR amplification / María Rosa Ponce [and others] -- Fluorescence in situ hybridization for the identification of environmental microbes / Annelie Pernthaler and Jakob Pernthaler -- Introduction to kinetic ("real-time") PCR / John Mackay -- Validation of short interfering RNA knockdowns by quantitative real-time PCR / Sukru Tuzmen, Jeff Kiefer, and Spyro Mousses.
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Real-time quantitative PCR as an alternative to southern blot or fluorescence in situ hybridization for detection of gene-copy number changes / Jasmien Hoebeeck, Frank Speleman, and Jo Vandesompele -- Design and work-up of a new molecular diagnostic assay based on real-time PCR / Harald H. Kessler -- Real-time PCR fluorescent chemistries / John Mackay and Olfert Landt -- Microarrays : an overview / Norman H. Lee and Alexander I. Saeed -- Oligonucleotide microarrays for the study of coastal microbial communities / Gaspar Taroncher-Oldenburg and Bess B. Ward.
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| Abstract
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Protocols for Nucleic Acid Analysis by Non-radioactive Probes, Second Edition provides a firm background on the basic preparative protocols required for the analysis of nucleic acids by nonradioactive methods. Presenting the methodologies using amazing new applications, this volume offers guide chapters on nucleic acid extractions, preparation of nucleic acid blots, and labeling of nucleic acids with nonradioactive haptens. New fluorescent techniques such as Real Time PCR and microarrays are also included, allowing users to get a nonradioactive protocol implemented in the laboratory with minimum adaptation required and fastest time to results. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
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| Subject
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Nucleic acid probes.
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Nucleic acids-- Analysis.
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DNA Probes.
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DNA-- analysis.
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Molecular Probe Techniques.
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Nucleic Acid Amplification Techniques.
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Nucleic acid probes.
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Nucleic acids-- Analysis.
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SCIENCE-- Life Sciences-- Genetics Genomics.
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DNA probes.
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DNA-- Analysis.
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Molecular Probe Techniques.
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Nucleic Acid Amplification Techniques.
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Nucleic acid probes.
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Nucleic acids-- Analysis.
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| Dewey Classification
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572.8/636
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| LC Classification
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QP620.P765 2007
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| NLM classification
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QU 58.5P9674 2007
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W1ME9616J v.353 2007
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Q520. 3clc
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| Added Entry
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Hilario, Elena,1966-
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Mackay, John,1969-
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