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" Hypertension : "
edited by Jérôme P. Fennell, Andrew H. Baker.
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BL
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| Record Number
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1047386
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b801756
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| Title & Author
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Hypertension : : methods and protocols /\ edited by Jérôme P. Fennell, Andrew H. Baker.
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| Publication Statement
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Totowa, N.J. :: Humana Press,, ©2005.
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| Series Statement
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Methods in molecular medicine,; 108
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| Page. NO
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1 online resource (xvi, 501 pages) :: illustrations
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| ISBN
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1280359641
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: 1588293238
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: 1592598501
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: 6610359644
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: 9781280359644
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: 9781588293237
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: 9781592598502
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: 9786610359646
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| Bibliographies/Indexes
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Includes bibliographical references and index.
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| Contents
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Construction and characterization of helper-dependent adenoviral vectors for sustained in vivo gene therapy / Kazuhiro Oka and Lawrence Chan -- Adenovirus-mediated gene transfer in vivo: an approach to reduce oxidative stress / Yi Chu -- Gene therapy for hypertension: antisense inhibition of the renin-angiotensin system / M. Ian Phillips and Birgitta Kimura -- Modulation of gene expression by RNAi / Cezary Wojcik, Rosalind Fabunmi, and George N. DeMartino -- In vivo biopanning: a methodological approach to identifying novel targeting ligands for delivery of biological agents to the vasculatre / Lorraine M. Work [and others] -- pt. VI. Stem cells. Cardiomyocytes derived from embryonic stem cells / Kenneth R. Boheler [and others] -- pt. VII. Bioinformatics. In Silico and wet-bench identification of nuclear matrix attachment regions / Stephen A. Krawetz [and others] -- Biomedical informatics methods in pharmacogenomics / Qing Yan.
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Denaturing high-performance liquid chromatography using the WAVE DNA fragment analysis system / Elizabeth Donohoe -- TaqMan real-time PCR quantification: conventional and modified methods / Michihiro Yoshimura, Shota Nakamura, and Hisao Ogawa -- Generation of high-sensitivity antisense cDNA probes by asymmetric PCR / Ian M. Bird -- New advances in microarrays: finding the genes causally involved in disease / Claudio Napoli, Filomena de Nigris, and Vincenzo Sica -- Pharmacogenetics and pharmacogenomics / Klaus Lindpaintner -- pt. IV. Proteins and proteomics. Determination of the endogenous nitric oxide synthase inhibitor asymmetric dimethylarginine in biological samples by HPLC / Tom Teerlink -- Proteomic approaches in the analysis of hypertension / Soren Naaby-Hansen [and others] -- pt. V. Gene transfer. Gene transfer in endothelial dysfunction and hypertension / Sabyasachi Sen, Padraig M. Strappe, and Timothy O'Brien -- Nonviral gene delivery methods in cardiovascular diseases / Marika Ruponen [and others].
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pt. I. Models of hypertension. Congenic/consomic models of hypertension / Delyth Graham [and others] -- Mouse knockout models of hypertension / Michael Bader -- Production of transgenic models in hypertension / Elena Popova, Michael Bader, and Alexander Krivokharchenko -- pt. II. Assessment of free radicals in endothelial dysfunction. Analysis of superoxide anion production in tissue / Michela Zanetti [and others] -- Measurement of vascular reactive oxygen species production by chemiluminescence / Tomasz J. Guzik and Keith M. Channon -- A guide to wire myography / Angela Spiers and Neal Padmanabhan -- pt. III. Nucleic acid techniques. Selection of cardidate genes in hypertension / Charles A. Mein, Mark J. Caulfield, and Patricia B. Munroe -- Genome-wide scanning with SSLPs in the rat / Carol Moreno [and others] -- Extraction of RNA from cells and tissue / Ian M. Bird -- Single-strand conformational polymorphism analysis: basic principles and routine practice / Yanbin Dong and Haidong Zhu -- Single-nucleotide polymorphism genotyping for disease association studies / Myriam Fornage and Peter A. Doris.
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| Abstract
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Our understanding of the pathogenesis of hypertension and the development of new therapies for its management have each proven to be directly dependent on the availability and application of novel molecular biological methodologies. In Hypertension: Methods and Protocols, a panel of recognized experts from key institutions around the world describes in step-by-step detail many new and essential molecular techniques for cardiovascular hypertension research. The methods presented range widely from producing congenic, consomic, transgenic, and knockout models of hypertension to the gene transfer of specific genetic material using adenoviral and nonviral (polymers, liposomes, and antisense agents) vectors. Additional techniques described include single nucleotide polymorphism (SNP) genotyping, RNA interference, microarray analysis, pharmacogenetics, and pharmacogenomics for the genetic dissection of hypertension, as well as a practical method for deriving cardiomyocytes from embryonic stem cells that would serve as replacement cells for those damaged by hypertension or heart attack. A review of bioinformatic resources for pharmacogenomics describes the application of in silico strategies to identify nuclear matrix attachment regions. The protocols follow the successful Methods in Molecular Medicine series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. State-of-the-art and highly practical, Hypertension: Methods and Protocols offers both novice and experienced hypertension researchers an indispensable collection of readily reproducible techniques for successful research, work that has already dramatically improved the outlook for hypertensive patients, and promises much future success.
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| Subject
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Hypertension-- Molecular aspects, Laboratory manuals.
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Hipertensão.
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MEDICAL-- Cardiology.
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Hypertension-- genetics.
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Clinical Protocols.
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Genetic Techniques.
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Hypertension-- therapy.
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Models, Animal.
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| Dewey Classification
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616.1/32
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| LC Classification
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RC685.H8H855 2005
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| NLM classification
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WG 340H99582 2005
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| Added Entry
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Baker, Andrew H.
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Fennell, Jérôme P.
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