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" UTILIDAD DE NANOPARTICOS MAGNÉTICOS PARA LA ENTREGA CELULAR ENDOTELIAL CORNEAL DIRECCIONADA "
Cornell, Lauren Elsworth
Glickman, Randolph D
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Latin Dissertation
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English
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| Record Number
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1051680
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| Doc. No
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TL50797
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| Main Entry
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Cornell, Lauren Elsworth
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| Title & Author
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UTILIDAD DE NANOPARTICOS MAGNÉTICOS PARA LA ENTREGA CELULAR ENDOTELIAL CORNEAL DIRECCIONADA\ Cornell, Lauren ElsworthGlickman, Randolph D
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| College
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The University of Texas Health Science Center at San Antonio
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| Date
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2019
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| Degree
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Ph.D.
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2019
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| Note
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85 p.
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| Abstract
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During military operations in Iraq and Afghanistan, 16% of battlefield evacuations were attributable to ocular trauma. Potential for corneal opacification and vision loss is elevated if the corneal endothelium is damaged. The cornea is the outer-most clear part of the eye which allows light to enter. To maintain this unique transparency, a single inner-layer of endothelial cells must properly exchange electrolytes and water within the cornea. If endothelial cell density drops below 500-1000 cells per mm2, then the cornea can become opaque, leading to vision loss. Unfortunately, these cells do not proliferate well, become lower in density with age, and require surgical procedures to be replaced. Here, we investigate the principle that magnetic nanoparticles can be pre-loaded into cultured endothelial cells, which may then be injected directly into the anterior chamber and used in conjunction with an external magnetic field to deliver them to the posterior of the cornea. This innovative technique would allow traumatized or diseased endothelium to be replaced using a minimally-invasive procedure. To this end, our project aims to develop the scientific methodology and perform “proof of concept” experiments that can provide the foundation warranting further in-depth studies. Specifically, through collaborative efforts, we aim to 1) grow and maintain corneal endothelial cell populations and determine impacts on viability of nanoparticle exposure 2) to further develop a magnetic-based technology for delivering these cells to the inner cornea using an ex vivo model system, 3) show attachment of cells to cornea.
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| Descriptor
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Nanotechnology
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Ophthalmology
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Glickman, Randolph D
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The University of Texas Health Science Center at San Antonio
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