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" Evaluation of Antioxidant Properties and Urease Inhibitory Activity of Methanolic Seed Extract of Aframomum melegueta, Piper guineense and Monodora myristica "
Abdulhammed, Hassan Taiye
Irondi, A. E.
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Latin Dissertation
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| Language of Document
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English
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| Record Number
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1052449
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TL51566
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| Main Entry
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Abdulhammed, Hassan Taiye
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| Title & Author
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Evaluation of Antioxidant Properties and Urease Inhibitory Activity of Methanolic Seed Extract of Aframomum melegueta, Piper guineense and Monodora myristica\ Abdulhammed, Hassan TaiyeIrondi, A. E.
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| College
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Kwara State University (Nigeria)
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| Date
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2019
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| Degree
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M.S.
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2019
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| Note
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108 p.
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| Abstract
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Spices are prominent for their diverse health benefits, which are often attributed to their bioactive components. Hence, this study evaluated bioactive components,antioxidant properties and urease inhibitory activityof methanolic extracts of Aframomummelegueta (AM), Piper guineense (PG) and Monodora myristica (MM) seeds, using spectrophotometer and gas chromatography couple with mass spectrophotometer. The phytochemicals screening revealed that PG had significantly higher (p<0.05) total phenols and flavonoid contents compare to MM and AM. The result of gas chromatography couple with mass spectrometer (GC-MS) also revealed that PG had higher bioactive components from the analysis compared to others and there is pronounce similarity in the bioactive components of PG and AM compare to PG and MM. The extracts effectively inhibited urease in a dose-dependent manner, having IC50 in the order of PG < MM < AM [PG (12.96 µg/mL), MM (13.83 µg/mL), AM (13.88 µg/mL) respectively. The listed antioxidant properties were measured, which include1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging ability, iron (II) chelation and reducing power (RP) on the extracts. Of the three spices, DPPH scavenging ability and iron (II) chelation of the seed extracts increased in a dose-dependent manner, having IC50 in the order of PG < AM < MM [PG (0.11 mg/mL), AM (0.16 mg/mL), MM (0.35 mg/mL)] and as well PG < MM < AM [PG (0.31 mg/mL), MM (0.35 mg/mL), AM (0.36 mg/mL] respectively. The study therefore, concludes that Piper guineense has better urease inhibitory effect and antioxidant activity than Monodora myristica and Aframomum melegueta. Thus, Piper guineense seed may serve as functional food for managing urolithiasis and attenuating the oxidative stress associate with the disease.
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| Descriptor
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Biochemistry
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Irondi, A. E.
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Kwara State University (Nigeria)
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