The Role of Mechanosensitive Ion Channel PIEZO1 in Detection of Confinements at Ecm

مرکز و کتابخانه مطالعات اسلامی به زبان های اروپایی

منو

" The Role of Mechanosensitive Ion Channel PIEZO1 in Detection of Confinements at Ecm "
Bahrani Fard, Mohammad Reza Hua, Susan Z.

Document Type	:	Latin Dissertation
Language of Document	:	English
Record Number	:	1052478
Doc. No	:	TL51595
Main Entry	:	Bahrani Fard, Mohammad Reza
Title & Author	:	The Role of Mechanosensitive Ion Channel PIEZO1 in Detection of Confinements at Ecm\ Bahrani Fard, Mohammad RezaHua, Susan Z.
College	:	State University of New York at Buffalo
Date	:	2019
Degree	:	M.S.
student score	:	2019
Note	:	57 p.
Abstract	:	Cellular response to confinement at the extracellular matrix (ECM) plays an important role during tissue development. Mishandling these mechanical signals results in a variety of diseases including cardiovascular disease and cancer. In this study, we investigate the role of mechanosensitive Piezo1 channel as a cell sensor for detection of mechanical cues at various substrates. We modified the substrates with various micropatterns, topographies, and stiffness and examined the cell shape in response to ECM modifications. By inhibiting Piezo1 activity with specific pharmacologic inhibitors or by knockout of Piezo1 with siRNA, we have shown for the first time that HEK cells lost their ability to stretch to the full extent on the stripe patterns compared with wild-type cells. This indicates that cells utilize Piezo1 channels for detection of substrate confinements. The location and concentration of Piezo1 channels were visualized using FRET-based fluorescent probes P1-cpstFRET. We show that the distribution of Piezo1 is non-uniform in cells on narrow stripes, and Piezo1 clusters exist in areas of high cytoskeletal tension. This work offers a new mechanism of mechanosensing by Piezo1 channels, through which cells utilize ECM cues for remodeling.
Descriptor	:	Bioengineering
	:	Cellular biology
	:	Mechanical engineering
Added Entry	:	Hua, Susan Z.
Added Entry	:	State University of New York at Buffalo