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" Antimicrobial Photodynamic Therapy in an ex-vivo Peri-Implantitis Model "
Jamaleddin, Mohammad Waseem
Mang, Thomas S.
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Latin Dissertation
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| Language of Document
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English
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| Record Number
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1053823
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| Doc. No
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TL52940
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| Main Entry
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Jamaleddin, Mohammad Waseem
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| Title & Author
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Antimicrobial Photodynamic Therapy in an ex-vivo Peri-Implantitis Model\ Jamaleddin, Mohammad WaseemMang, Thomas S.
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| College
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State University of New York at Buffalo
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| Date
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2020
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| Degree
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M.S.
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2020
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| Note
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59 p.
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| Abstract
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Background: Implant surface disinfection is the most challenging aspect of treating peri-implantitis with no established consensus despite having a lot of research on a host of various techniques ranging from mechanical to chemical to laser treatments. Antimicrobial photodynamic therapy (PDT) is a promising technique that uses light in the presence of a non-toxic photosensitizer to induce the formation of reactive oxygen species (ROS) for disinfection. The purpose of this study is to evaluate the effect of antimicrobial photodynamic therapy on 48 hour planktonic and biofilm cultures of Staphylococcus aureus (S. aureus) to investigate the killing efficacy as well as determine the effect of drug incubation time and light dose on the efficacy of this treatment modality. Materials and methods: S. aureus was cultured for 48 hours and then inoculated at an optical density (OD) of 0.1 in phosphate-buffered saline. PDT was carried out using porfimer sodium (Photofrin®) with a 630 nm Diode laser. Light doses of 75 and 100 J/cm2 were used at 150 mW/cm2, and the photosensitizer (125μg/mL) with incubation times varying from 5, 10, and 15 minutes. The control groups were no light and no drug, drug only, and laser only. Bacterial survival was assessed by CFU count after plating in TSA with a dilution of 1:10000 and incubation of 48 hours. For PDT on biofilm of S. aureus, bovine bone blocks were used. Implants were placed along with a simulated peri-implant defect exposing implant threads. S. aureus was grown in TSA for 48 hours and then incubated in the defect with TSB. Biofilm growth was allowed for two days in the models before proceeding with the experiment. Light doses were 150 and 200J/cm2 at 200 mW/cm2 with photosensitizer (250 μg/mL) incubation times of 30 and 60 minutes used. Bacterial survival was assessed with CFU count, and the retrieval was accomplished by washing the defect three times with TSB. Results: Initial planktonic experiments showed the susceptibility of S. aureus to PDT. The antimicrobial efficacy ranged from 7 to 2 log kill. There was no statistically significant difference in antimicrobial efficacy by incubation time or light dose used except in the 5min/75J combination, which showed no significant difference with the negative control. The biofilm experiment showed good growth of bacteria in control groups and a statistically significant bacterial kill in the experimental groups with no difference in the incubation times or the light dose used. Conclusion: This study demonstrates the effectiveness of PDT in decontaminating a simulated peri-implant defect and on planktonic S.aureus colonies. Further studies are still needed to assess the efficacy of PDT on a multispecies bacterial biofilm and determine optimal light doses, drug concentrations, and incubation time.
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| Descriptor
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Dentistry
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Mang, Thomas S.
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State University of New York at Buffalo
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