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" Molecular Surveillance of Plasmodium Falciparum Chloroquine Resistant Transporter Genes Among Selected Secondary School Students in Ilorin East L.G.A, Kwara State "
Hadiza, Ismail Onono
Olalubi, O.
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Latin Dissertation
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| Language of Document
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English
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| Record Number
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1054459
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TL53576
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| Main Entry
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Hadiza, Ismail Onono
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| Title & Author
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Molecular Surveillance of Plasmodium Falciparum Chloroquine Resistant Transporter Genes Among Selected Secondary School Students in Ilorin East L.G.A, Kwara State\ Hadiza, Ismail OnonoOlalubi, O.
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| College
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Kwara State University (Nigeria)
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| Date
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2019
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| Degree
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M.S.
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2019
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| Note
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120 p.
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| Abstract
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High transmission rate and drug resistance have been linked to the spread and re-emergence of malaria. Mutations in the Plasmodium falciparum chloroquine resistance transporter (Pfcrt), are the major determinant of chloroquine resistance in human malaria parasite. Plasmodium falciparum malaria as well as Pfcrt and Pfmdr1 genes of isolates among secondary school students within the age range of 11-18 years from three selected rural communities of Kwara State were studied. One hundred and eighty seven subjects (187) were selected for the study. Scientific and Ethical clearance was obtained from the ethical review committee for research in human subjects, Ministry of Public Health, Kwara State. Blood samples were collected by finger prick method for malaria screening while structured questionnaires were administered to extract information from the subjects. DNA of isolates were appropriately extracted from filter paper blots using the methanol fixation method. The extracted DNA was amplified and sorted out by PCR and Gel electrophoresis. The alleles at codon 76 and 86 of Pfcrt and Pfmdr1 genes of the isolates were equally studied. Logistic regression was performed on the binary observations obtained while linear regression was conducted on the fifty subjects that tested positive to malaria. Out of 187 subjects screened, 26.7% (50) were positive to falciparum malaria. Highest malaria count of 36.4% was recorded among subjects of 13-15years. With 0.337 as the p-value for the coefficient of age (greater than significant level) obtained from linear regression, the study revealed that age is not statistically significant in explaining parasite density. The result of the studied isolates indicated that out of 50 isolates analyzed for Pfcrt gene, wild type alleles accounted for 32% (16) isolates while mutant alleles accounted for 68 % (34) isolates. Alakuko Community accounted for the least number of T76 mutant alleles 14% (7) isolates while Apado community indicated the highest number of T76 mutant gene 28% (14) isolates. For Pfmdr1 gene analysis at codon 86, isolates from Apado community showed the highest mutant alleles (Y86) of 24% (12) isoletes, while Alakuko community gave the least mutant alleles, 14% (6) isoletes. The overall result revealed existence of mutant alleles in both the Pfcrt and Pfmdr1 genes which was higher than the wild type gene in both cases. The prevalence of the mutant genes over the wild type genes from the study indicated the availability of chloroquine resistance genes among the studied population.
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| Descriptor
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Epidemiology
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Genetics
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Immunology
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Pathology
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Zoology
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| Added Entry
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Olalubi, O.
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| Added Entry
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Kwara State University (Nigeria)
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