|
" Deri̇ Doku Mühendi̇sli̇ği̇ Uygulamalarina Yöneli̇k Halomonas Levan Hi̇drojelleri̇ni̇n Geli̇şti̇ri̇lmesi̇ "
Selvi, Sinem Selvin
Öner, Ebru Toksoy
| Document Type
|
:
|
Latin Dissertation
|
| Language of Document
|
:
|
English
|
| Record Number
|
:
|
1055586
|
| Doc. No
|
:
|
TL54703
|
| Main Entry
|
:
|
Selvi, Sinem Selvin
|
| Title & Author
|
:
|
Deri̇ Doku Mühendi̇sli̇ği̇ Uygulamalarina Yöneli̇k Halomonas Levan Hi̇drojelleri̇ni̇n Geli̇şti̇ri̇lmesi̇\ Selvi, Sinem SelvinÖner, Ebru Toksoy
|
| College
|
:
|
Marmara Universitesi (Turkey)
|
| Date
|
:
|
2019
|
| Degree
|
:
|
Master's
|
| student score
|
:
|
2019
|
| Note
|
:
|
92 p.
|
| Abstract
|
:
|
Hydrogels have high hydrophilic features and they are synthesized by crosslinking with chemical and physical methods. They are 3-dimensional structures with great water containing capacity. These polymeric networks are able to swell and de-swell in reversible ways. Hydrogels are recently widely used in cell, tissue and organ repair and regenerations. Several different polymers are used for hydrogel formation. Levan is fructose polymer that consist of β (2 → 6) linkages. Levan is a bio-compatible, water soluble and highly adhesive polysaccharide and it is widely used in medicine, food and cosmetic industry but researches are limited due to high costs of production. Halomonas smyrnensis is reported as the first halophilic levan producer extremophile and this microbial system decreased the production costs considerably by enabling unsterile production conditions. In further years, many studies such as anti-cancer, anti-oxidant and anti-coagulant features of levan were investigated. Within the scope of the thesis, levan is aimed to be used in skin tissue engineering. Levan is first hydrolyzed and then modified. Phosphonation, sulfation and phosphonation of sulfated levan modifications were performed. Modifications were structurally verified with FTIR and EDS. Natural Halomonas levan (HL) and modified levan polysaccharides were crosslinked with BDDE in order to form hydrogels. Only HL and phosphonated levan (PhHL) formed hydrogels with suitable properties. Swelling tests of synthesized hydrogels were performed and highest swelling degree was found to be 5.092 (in PBS, at 37oC) which belonged to HL hydrogel with lower crosslinker amount. Due to high amount of BDDE, swelling degrees of PhHL hydrogels were found to be lower. In further studies, resveratrol entrapment and release studies were determined. HL hydrogel with less BDDE, released nearly all resveratrol within only 6 hours. PhHL1 hydrogel released 83.4% after 24 hours and no further release was found after 72 hours. Rheological studies and adhesive properties were investigated with rheometer. Also, cell culture experiments were performed with human keratinocyte cells. After 72 hours it was found that all hydrogels were biocompatible and suitable for skin tissue engineering applications.
|
| Descriptor
|
:
|
Bioengineering
|
|
|
:
|
Biomedical engineering
|
| Added Entry
|
:
|
Öner, Ebru Toksoy
|
| Added Entry
|
:
|
Marmara Universitesi (Turkey)
|
| |