|
" Modulation of energy homeostasis in maize and Arabidopsis to develop lines tolerant to drought, genotoxic and oxidative stresses "
Elizabeth Njuguna, Griet Coussens, Stijn Aesaert, et al.
| Document Type
|
:
|
AL
|
| Record Number
|
:
|
1064204
|
| Doc. No
|
:
|
LA107833
|
| Call No
|
:
|
10.1163/2031356X-03002007
|
| Language of Document
|
:
|
English
|
| Main Entry
|
:
|
Elizabeth Njuguna
|
|
|
:
|
Griet Coussens
|
|
|
:
|
Mieke Van Lijsebettens
|
|
|
:
|
Pia Neyt
|
|
|
:
|
Stijn Aesaert
|
|
|
:
|
Sylvester Anami
|
| Title & Author
|
:
|
Modulation of energy homeostasis in maize and Arabidopsis to develop lines tolerant to drought, genotoxic and oxidative stresses [Article]\ Elizabeth Njuguna, Griet Coussens, Stijn Aesaert, et al.
|
| Publication Statement
|
:
|
Leiden: Brill
|
| Title of Periodical
|
:
|
Afrika Focus
|
| Date
|
:
|
2017
|
| Volume/ Issue Number
|
:
|
30/2
|
| Page No
|
:
|
66–76
|
| Abstract
|
:
|
Abiotic stresses cause crop losses worldwide that reduce the average yield by more than 50%. Due to the high energy consumed to enhance the respiration rates, the excessive reactive oxygen species release provokes cell death and, ultimately, whole plant decay. A metabolic engineering approach in maize (Zea mays) altered the expression of two poly(ADP-ribosyl)ation metabolic pathway proteins, poly(ADP-ribose) polymerase (PARP) and ADP-ribose-specific Nudix hydrolase (NUDX) genes that play a role in the maintenance of the energy homeostasis during stresses. By means of RNAi hairpin silencing and CRJSPR/Cas9 gene editing strategies, the PARP expression in maize was downregulated or knocked down. The Arabidopsis NUDX7 gene and its two maize homologs, ZmNUDX2 and ZmNUDX8, were overexpressed in maize and Arabidopsis. Novel phenotypes were observed, such as significant tolerance to oxidative stress and improved yield in Arabidopsis and a trend of tolerance to mild drought stress in maize and in Arabidopsis. Abiotic stresses cause crop losses worldwide that reduce the average yield by more than 50%. Due to the high energy consumed to enhance the respiration rates, the excessive reactive oxygen species release provokes cell death and, ultimately, whole plant decay. A metabolic engineering approach in maize (Zea mays) altered the expression of two poly(ADP-ribosyl)ation metabolic pathway proteins, poly(ADP-ribose) polymerase (PARP) and ADP-ribose-specific Nudix hydrolase (NUDX) genes that play a role in the maintenance of the energy homeostasis during stresses. By means of RNAi hairpin silencing and CRJSPR/Cas9 gene editing strategies, the PARP expression in maize was downregulated or knocked down. The Arabidopsis NUDX7 gene and its two maize homologs, ZmNUDX2 and ZmNUDX8, were overexpressed in maize and Arabidopsis. Novel phenotypes were observed, such as significant tolerance to oxidative stress and improved yield in Arabidopsis and a trend of tolerance to mild drought stress in maize and in Arabidopsis.
|
| Descriptor
|
:
|
CRISPR/Cas9
|
| Descriptor
|
:
|
drought stress
|
| Descriptor
|
:
|
maize
|
| Descriptor
|
:
|
Nudix hydrolase
|
| Descriptor
|
:
|
oxidative stress
|
| Descriptor
|
:
|
poly(ADP-ribose) polymerase
|
| Location & Call number
|
:
|
10.1163/2031356X-03002007
|
| |