|
" Characterization and Partial Purification of Pectinase Produced by Aspergillus niger Using Banana Peel as Carbon Source "
Yusuf, Ahmed Olaitan
Adedayo, M. R.
| Document Type
|
:
|
Latin Dissertation
|
| Language of Document
|
:
|
English
|
| Record Number
|
:
|
1106328
|
| Doc. No
|
:
|
TLpq2385396538
|
| Main Entry
|
:
|
Adedayo, M. R.
|
|
|
:
|
Yusuf, Ahmed Olaitan
|
| Title & Author
|
:
|
Characterization and Partial Purification of Pectinase Produced by Aspergillus niger Using Banana Peel as Carbon Source\ Yusuf, Ahmed OlaitanAdedayo, M. R.
|
| College
|
:
|
Kwara State University (Nigeria)
|
| Date
|
:
|
2019
|
| student score
|
:
|
2019
|
| Degree
|
:
|
M.S.
|
| Page No
|
:
|
59
|
| Abstract
|
:
|
Pectinases are group of enzymes which causes the degradation of pectin. Pectinases are of industrial importance due to their biotechnological and commercial potentials in brewing industries, textile industries, food industries and paper industries among others. The present study was aimed at characterization and partial purification of pectinase produced by Aspergillus niger using banana peel as carbon source under submerged fermentation (SmF) system. Pectinolytic fungi were isolated from fruit wastes dumping sites. The isolates were characterized morphologically and microscopically using standard methods. The isolates were further subjected to screening for pectinolytic ability. Molecular identification was also carried out on the isolate with the best pectinolytic ability. The isolate with the best pectinolytic ability was identified as A. niger strain AH1 with GenBank accession number MK811422. Fermentation parameters optimized to increase pectinase activity include incubation period, salt supplements, additional carbon source, inoculum sizes, pH, moisture contents and temperature using standard methods. The crude pectinase obtained was purified by ammonium sulphate precipitation, dialysis and gel-filtration chromatography. Maximum pectinase activity of 575.37±0.67 U/mg/min/ml was obtained at day 3 of incubation, with addition of salt supplements, in the presence of sucrose as additional carbon source, 2 ml of 1 × 106 spores ml-1 of A. niger, pH 6, 100 ml moisture contents, and temperature of 50 0C. The specific activity increased with 1.38 fold while recovery yield was 8.94 %. The study confirmed that the isolated A. niger strain AH1 can produced maximum pectinase at optimized fermentation parameters and could be explored for pectinase production.
|
| Subject
|
:
|
Microbiology
|
| |