A Study of Bacterial Translation at Codon Resolution Using Ribosome Profiling

منو

" A Study of Bacterial Translation at Codon Resolution Using Ribosome Profiling "
Mohammad, Fuad Nathans, Jeremy

Document Type	:	Latin Dissertation
Language of Document	:	English
Record Number	:	1107296
Doc. No	:	TLpq2439936824
Main Entry	:	Mohammad, Fuad
	:	Nathans, Jeremy
Title & Author	:	A Study of Bacterial Translation at Codon Resolution Using Ribosome Profiling\ Mohammad, FuadNathans, Jeremy
College	:	The Johns Hopkins University
Date	:	2020
student score	:	2020
Degree	:	Ph.D.
Page No	:	178
Abstract	:	Ribosome profiling has pushed the boundary of how translation is studied by illuminating every step of the translation cycle at the genome scale. First developed by Nick Ingolia and Jonathan Weismann, ribosome profiling is now widely used in both bacterial and eukaryotic studies. However, development of the method in bacteria has not achieved the level of refinement seen in yeast and mammalian ribosome profiling. This thesis focuses on analyzing the current methodology in bacteria to understand its strengths and shortcomings and developing improvements in both how libraries are prepared and how the data is analyzed. This thesis will also focus on implementing these improvements to understand events that influence translation elongation as well as how ribosome profiling can be used to identify new genes.
Subject	:	Bioinformatics
	:	Molecular biology