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" Molecular Investigation of Anaerobic Ammonium Oxidation (Anammox) Bacteria Under Long-Term Nanoparticle Exposure "
Sarı, Tuğba
Akgül, Deniz
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Latin Dissertation
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| Language of Document
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English
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| Record Number
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1109684
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| Doc. No
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TLpq2476160197
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| Main Entry
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Akgül, Deniz
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Sarı, Tuğba
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| Title & Author
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Molecular Investigation of Anaerobic Ammonium Oxidation (Anammox) Bacteria Under Long-Term Nanoparticle Exposure\ Sarı, TuğbaAkgül, Deniz
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| College
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Marmara Universitesi (Turkey)
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| Date
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2019
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| student score
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2019
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| Degree
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Master's
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| Page No
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157
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| Abstract
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Anammox has become an innovative bioprocess in wastewater treatment facilities due to its significant advantages over conventional nitrification/denitrification process. However, variable responses of anammox bacteria to external environment and slow growth rate of them cause limitations to industrialization of this process. Besides, increased utilization of nanoparticles worldwide and releasing of them into the ecosystem, notably in wastewater treatment plants, lead to great concern about their adverse impacts on biological systems. In this context, the long-term inhibitive effects of ZnO and TiO2 nanoparticles on bench-scale anammox systems were individually investigated in terms of nitrogen removal performance, metal analysis, assessment of EPS and microbial diversity within the bioreactor. ZnO nanoparticles showed more toxic effects at a lower concentration in less time than TiO2 nanoparticles. Although similar profiles on EPS secretion were observed, higher amount of EPS was produced by microbial consortia exposed to TiO2 nanoparticles. According to qPCR, anammox population firstly decreased up to a certain concentration for both nanoparticles. Subsequently, relative anammox abundance increased with the adaptation to stress factor and decreased again due to inhibition. 86% reduction on relative anammox quantification was observed during TiO2 nanoparticles exposure at the inhibition concentration. As for ZnO nanoparticles exposure, the metagenomic analysis revealed that relative abundance of Ca. Brocadiacea family was approximately halved which was consistent with qPCR. Lastly, overall biodiversity slightly changed in both bioreactors although various shifts in microbial consortia were detected according to DGGE.
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Bioengineering
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Civil engineering
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Nanoscience
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