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" In-Vivo Studies of the Trypanocidal and Toxicity Activities of Aframomum melegueta (Alligator Pepper) "
Ogunlusi, Blessing Omolara
Ojo, Sunday
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Latin Dissertation
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| Language of Document
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English
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| Record Number
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1112100
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| Doc. No
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TLpq2516283728
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| Main Entry
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Ogunlusi, Blessing Omolara
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Ojo, Sunday
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| Title & Author
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In-Vivo Studies of the Trypanocidal and Toxicity Activities of Aframomum melegueta (Alligator Pepper)\ Ogunlusi, Blessing OmolaraOjo, Sunday
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| College
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Kwara State University (Nigeria)
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| Date
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2021
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| student score
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2021
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| Degree
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M.S.
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| Page No
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149
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| Abstract
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Trypanosomiasis is a disease caused by a bite from an infected tsetse fly and infect ruminant animals and also human. Herbal medicines are popularly used remedies for many diseases by a vast majority of the world’s population, because allopathic medicines have large number of side-effects. It is in view of this that this study evaluates the in-vivo studies of the Trypanocidal and toxicity activities of Aframomum melegueta (Alligator Pepper). Seeds of Aframomum melegueta were sundried and grounded. The powdered seed was used for phytochemical analysis using methanol under standard procedures, to determine both the quantitative and qualitative component of the seed. Rats for the experiment were gotten from the animal unit of the Biochemistry Department of University of Ilorin, and were infected with Trypanosoma brucei brucei parasite obtained from stabilates maintained at the Nigerian Institute for Trypanosomiasis Research (NITR), Vom, Plateau State, Nigeria through transverse passage. Thirty rats were used, and were grouped into six groups, (group A-F) and were inoculated intraperitoneally with trypanosomes (2.5×104). The rats were screened for trypanosomes three days post infection using rapid matching counting method. When parasitaemia count was 5×108 trypanosomes/ml on day 9 post infection. Rats in Group A–E were treated with 0.1 ml, 0.2 mls, 0.3 mls, 0.4 mls and 0.5 msl of methanolic seed extract of Aframomum melegueta through the oral route while Group F infected untreated (control group) received normal saline alone all through. Rats were monitored daily, during and after treatment for Parasitaemia and packed cell volume (PCV) for 60 days. Dead rats were disserted and the liver examined for histopathology. Results reveal that Aframomum melegueta contains compounds which make it a promising antitrypanocidal medicine but with a damaging effect on the liver of rats which could result to their mortality if consumed in large quanties.
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Zoology
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