İnsanda apoptozu düzenleyen uzun kodlamayan RNA'ların moleküler karakterizasyonu

مرکز و کتابخانه مطالعات اسلامی به زبان های اروپایی

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" İnsanda apoptozu düzenleyen uzun kodlamayan RNA'ların moleküler karakterizasyonu "
Sweef, Osama Akgül, Bünyamin

Document Type	:	Latin Dissertation
Language of Document	:	English
Record Number	:	1112304
Doc. No	:	TLpq2524377669
Main Entry	:	Akgül, Bünyamin
	:	Sweef, Osama
Title & Author	:	İnsanda apoptozu düzenleyen uzun kodlamayan RNA'ların moleküler karakterizasyonu\ Sweef, OsamaAkgül, Bünyamin
College	:	Izmir Institute of Technology (Turkey)
Date	:	2019
student score	:	2019
Degree	:	Ph.D.
Page No	:	149
Abstract	:	Apoptosis is an evolutionarily form of programmed cell death for development and tissue homeostasis. Apoptosis is regulated by protein-coding genes and plays an important role in a wide range of biological processes. We aimed to identify and characterize differentially expressed lncRNAs in apoptosis. HeLa cells were used as a model system to identify the lncRNAs. The total RNAs was subjected to deep sequencing by next-generation sequencing. OmicsBOX Bioinformatics tools were used for differential expression analysis of lncRNAs that are apoptosis-induced. Gene set enrichment analysis (GSEA) was used to profile the miRNAs targeting lncRNAs. Cytoscape software was used to reconstruct lncRNA-miRNA targeting networks. RT-qPCR was used to validate miRNAs and their targets of lncRNAs and it was found that the overexpression of miR-519d-3p causes downregulation of lncRNAs RAB22A-202, PARD3-211, and AC027237.1-210. Also, the overexpression of miR-124-3p down-regulates the expression level of APEX2-202 and CD59-209. GTF2A1-AS, TNFRSF10B-AS, and CAMTA1-DT were detected in the nucleus and have no poly (A) tail and they belong to TATA-less promoter genes. TNFRSF10B-AS has a coding probability of 0.99 and alignment to High-scoring Segment Pair (HSP) clarifies one hit to Q9UBN6 protein. ChIRP clarifies that TNFRSF10B-AS binds to a protein (25 kDa). miR-519d-3p and miR-124-3p interact with lncRNA targets by miRNA-mediated lncRNA degradation pattern under apoptosis conditions. TNFRSF10B-AS has a putative regulatory function in the nucleus during apoptosis via binding specifically to the ribonucleoprotein partner.
Subject	:	Apoptosis
	:	Genetics
	:	Molecular biology