Further characterization of tRNA(Trp) genes of Saccharomyces cerevisiae

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" Further characterization of tRNA(Trp) genes of Saccharomyces cerevisiae "
M. A. Riazi

Document Type	:	Latin Dissertation
Language of Document	:	English
Record Number	:	1112611
Doc. No	:	TLpq304057231
Main Entry	:	M. A. Riazi
Title & Author	:	Further characterization of tRNA(Trp) genes of Saccharomyces cerevisiae\ M. A. Riazi
College	:	University of Alberta (Canada)
Date	:	1992
student score	:	1992
Degree	:	M.Sc.
Page No	:	118
Abstract	:	Two goals were pursued in this work. First, attempts were made to construct an intronless ochre suppressor tRNATrp gene. The ability of the construct to function as an ochre suppressor was studied in Saccharomyces cerevisiae in vivo. The in vitro-constructed intronless tRNATrp gene suppressed the tested ochre mutations. This result supported models emphasizing the importance of the intron-anticodon stem loop binding in the splicing of pre-tRNA transcripts. These interactions are supposedly disrupted after a CCA to TTA change in the anticodon of the putative suppressor tRNATrp gene. An intronless gene would not have this problem since the need for splicing is abolished. The other interesting aspect of this work was the effect of an extrachromosomal factor (usd\Psi\sp{+}usd). The ability of the constructed tRNATrp gene to suppress one of the tested ochre mutations (lys1-1) was significantly increased in the presence of this factor, and reduced when this factor was eliminated. In the second part of this work the 5 and 3 flanking regions of the six tRNATrp genes in Saccharomyces cerevisiae were studied. (Abstract shortened by UMI.)
Subject	:	Biological sciences
	:	Genetics