Effects of ammonia, sulfur and isoacids on in vitro ruminal fermentation of tropical forages

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" Effects of ammonia, sulfur and isoacids on in vitro ruminal fermentation of tropical forages "
S. A. Rodriguez-Medina R. M. Cook

Document Type	:	Latin Dissertation
Language of Document	:	English
Record Number	:	1112665
Doc. No	:	TLpq303972303
Main Entry	:	R. M. Cook
	:	S. A. Rodriguez-Medina
Title & Author	:	Effects of ammonia, sulfur and isoacids on in vitro ruminal fermentation of tropical forages\ S. A. Rodriguez-MedinaR. M. Cook
College	:	Michigan State University
Date	:	1992
student score	:	1992
Degree	:	M.S.
Page No	:	108
Abstract	:	The effects of two levels of NH3, sulfur and isoacids on in vitro ruminal true digestibility (IVTD) of eight tropical forages was studied using a 2 factorial design. Trypticase was added to the final incubation media. This casein hydrolyzate should not have been added because it contributes NH3, isoacids and sulfur. This raised the levels of NH3 from the expected 5-10 to 17-26 mg/dl and isoacid levels about 3-fold. Sulfur levels were not affected. The results are discussed in light of trypticase addition. After a 48 h in vitro ruminal fermentation, NDF, ADF and lignin were measured to estimate IVTD. The results showed that IVTD increased with high levels of NH3 and when isoacids were added to treatments low in NH3. IVTD decreased when both NH3 and isoacids were high. High levels of sulfur tended to decrease IVTD for all forages, except elephant grass. Addition of isoacids increased total VFA concentration.
Subject	:	Biological sciences
	:	Livestock