Developmental patterns of tissue ornithine decarboxylase activity and nucleic acid concentrations in...

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" Developmental patterns of tissue ornithine decarboxylase activity and nucleic acid concentrations in lines of turkeys differing in body weight and breast muscle development "
D. A. Emmerson K. E. Nestor

Document Type	:	Latin Dissertation
Language of Document	:	English
Record Number	:	1112686
Doc. No	:	TLpq304000714
Main Entry	:	D. A. Emmerson
	:	K. E. Nestor
Title & Author	:	Developmental patterns of tissue ornithine decarboxylase activity and nucleic acid concentrations in lines of turkeys differing in body weight and breast muscle development\ D. A. EmmersonK. E. Nestor
College	:	The Ohio State University
Date	:	1991
student score	:	1991
Degree	:	Ph.D.
Page No	:	161
Abstract	:	Developmental patterns of tissue ornithine decarboxylase (OCD) activity and nucleic acid concentrations were evaluated in breast and leg muscle in a randombred control population (RBC2), a line selected for increased 16-week body weight (F), and a commercial sire line selected for increased body weight in conjunction with increased breast muscle development (C). Line C had similar body weight, but greater breast muscle development than F, while C and F both had greater body weights than the RBC2 population. Under standard assay conditions, ODC activity demonstrated linearity across time for more than five hours and had an apparent km of.22 mM. Tissue ODC activity reached its highest level on day 2 post-hatch and declined to relatively low basal levels by 35 days of age. Tissue ODC activity was greater for C than for F and RBC2 at 2 and 8 days of age, but there were no line differences at the other ages tested. The DNA content of muscle tissue was highest at hatch and declined thereafter. Breast muscle had a higher DNA content at hatch than leg muscle but this pattern was reversed after 8 days of age. Muscle DNA content was higher for the RBC2 population than for Lines F and C. Although muscle RNA content was similar across lines, the RNA content of breast muscle was greater than for leg muscle during the early post-hatch period. These results demonstrate changes in muscle functional cellularity in association with genetic increases in body weight, but not breast muscle development and modification of tissue ODC activity in association with increases in breast muscle mass, but not body weight. The influence of short-term fasting and refeeding on the ODC activity of breast muscle, liver, and intestinal tissue was also examined. While fasting produced large decreases in the ODC activity of all tissues examined, ODC activity rebounded quickly following refeeding. Compensatory increases in ODC activity were observed in liver and intestinal tissue. The ODC activity of breast muscle increased gradually to control values following refeeding. The results of this experiment demonstrated differential responses in ODC activity in breast muscle, liver, and intestinal tissue with fasting and refeeding and compensatory increases in liver and intestinal ODC activity with refeeding.
Subject	:	Anatomy physiology
	:	Animals
	:	Biological sciences
	:	Genetics
	:	Genetics
	:	Livestock