Micropropagation of 'John Franklin' rose and its phosphorus uptake

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" Micropropagation of 'John Franklin' rose and its phosphorus uptake "
J. Abdulnour N. N. Barthakur

Document Type	:	Latin Dissertation
Language of Document	:	English
Record Number	:	1112826
Doc. No	:	TLpq89188434
Main Entry	:	J. Abdulnour
	:	N. N. Barthakur
Title & Author	:	Micropropagation of 'John Franklin' rose and its phosphorus uptake\ J. AbdulnourN. N. Barthakur
College	:	McGill University (Canada)
Date	:	1993
student score	:	1993
Degree	:	M.Sc.
Page No	:	79
Abstract	:	Nodal sections of the winter-hardy 'John Franklin' rose cultivar from field-grown plants were cultured on a modified Murashige and Skoog (MS) nutrient medium. Very high levels of contamination from the surface of the initial sections required that plants be grown under greenhouse conditions. Rose plantlets obtained from subsequent subcultures were used for the first time in a radiotracer experiment with P to study the kinetics of phosphorus (P) uptake as a function of temperature of the nutrient medium. P uptake increased with time for rooted and non-rooted plantlets in a linear fashion that did not reach an equilibrium value even after 96 hours of exposure. An analysis of variance revealed that the plantlets with roots absorbed significantly greater amounts of P at the 0.01 level compared with non-rooted plantlets at 22C. P uptake was significantly higher at the 0.05 level, for rooted versus non-rooted plantlets at 33C. There was no significant difference in P uptake by rooted and non-rooted plantlets at 3C. Interaction between time of exposures and rooting was found to be significant at 22C and 33C at the 0.01 level. The results indicated that the root system, previously thought to be inefficient in the nutrient absorption, played a key role in absorbing P from the nutrient medium at optimum temperature.
Subject	:	Biological sciences
	:	Botany