Mycobacterium bovis infection in cattle in Morocco:

مرکز و کتابخانه مطالعات اسلامی به زبان های اروپایی

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" Mycobacterium bovis infection in cattle in Morocco: "
J. Berrada C. O. Thoen

Document Type	:	Latin Dissertation
Language of Document	:	English
Record Number	:	1112939
Doc. No	:	TLpq304049726
Main Entry	:	C. O. Thoen
	:	J. Berrada
Title & Author	:	Mycobacterium bovis infection in cattle in Morocco:\ J. BerradaC. O. Thoen
College	:	Iowa State University
Date	:	1993
student score	:	1993
Degree	:	Ph.D.
Page No	:	201
Abstract	:	Sodium deoxycholate (DOC) was utilized to extract immunologically-active cell wall components of virulent M. bovis and M. avium, isolated from cattle in Morocco and M. fortuitum as well as related organisms e.g., N. asteroides and R. equi. The extracts from M. bovis, N. asteroides, M. fortuitum, and M. avium, were fractionated by size exclusion chromatography using Sephadex S-200. Two fractions (1 and 2) were obtained at absorbance of 280 nm. Fraction 1 of M. bovis, M. avium, M. fortuitum and of N. asteroides and nonfractionated DOC extract of R. equi elicited delayed-type hypersensitivity responses in homologously or heterologously sensitized guinea-pigs. Significant enzyme-linked immunosorbent assay values were obtained using sera from cattle 4 weeks after sensitization with homologous bacterial preparations (p < 0.01). Significant ELISA cross-reactions were observed in M. bovis or M. avium sensitized calves, using heterologous antigens. Mycobacteriologic examinations conducted on tissues collected from 246 cows in 5 major abattoirs of Morocco, revealed that M. bovis was isolated from 29.67% of the cattle examined. Mycobacteria other than M. bovis (MOTT) were isolated from tissues of 23 carcasses (9.34%). Three had lesions compatible with tuberculosis; M. bovis was not isolated. An indirect enzyme-linked immunosorbent assay using M. bovis PPD or M. bovis DOC fraction 1 as antigens was used for detecting antibodies in sera of cattle from which M. bovis was isolated. The sensitivity for M. bovis PPD was 76.71% and for DOC extract of M. bovis was 79.45%; the specificity was of 90% and 92%, respectively. These values are similar to those achieved by the tuberculin skin test (71). Investigations conducted on five selected herds in Morocco using the cervical intradermal tuberculin test and ELISA revealed that cattle in three herds were tuberculous. The comparative cervical tuberculin test using M. bovis and M. avium PPD's at equal concentration was conducted within 24 hours of a positive or suspect skin response; no reactors were identified in the remaining two herds. Combination of ELISA and tuberculin skin test conducted on animals from tuberculous herds was found to be more sensitive than either test conducted alone.
Subject	:	Biological sciences
	:	immune response
	:	Microbiology
	:	Microbiology
	:	Veterinary services