Novel monoclonal antibodies against mouse NK cell receptors

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منو

" Novel monoclonal antibodies against mouse NK cell receptors "
L. Corral D. H. Raulet

Document Type	:	Latin Dissertation
Language of Document	:	English
Record Number	:	1112977
Doc. No	:	TLpq304347023
Main Entry	:	D. H. Raulet
	:	L. Corral
Title & Author	:	Novel monoclonal antibodies against mouse NK cell receptors\ L. CorralD. H. Raulet
College	:	University of California, Berkeley
Date	:	1997
student score	:	1997
Degree	:	Ph.D.
Page No	:	114
Abstract	:	Natural killer (NK) cells mediate the lysis of tumor cells, virally infected cells and bone marrow cell grafts. Whether these cells are killed by NK cells depends on a delicate balance between NK cell activating and inhibitory receptors. It has been demonstrated that cells with little or no cell surface expression of major histocompatibility complex (MHC) class I molecules are susceptible to NK cell lysis. However, there are MHC class I-deficient cells that are not lysed by NK cells. This indicates that additional activating signals may be required to induce NK cell lysis. NK cell activating receptors have not been very well characterized. To identify NK cell activating receptors that might be involved in tumor cell lysis, monoclonal antibodies (mAbs) were made in Syrian hamsters. Four mAbs generated were selected for further study. Two of the mAbs, 14B11 and 2F1, were specific for NK cells while the other two mAbs stained NK and T cells. The focus of this dissertation is on 14B11 and 2F1 mAbs. 14B11 mAb recognizes both Ly49C and Ly49I, MHC class I-specific inhibitory receptors expressed on NK cells. 14B11 mAb recognizes at least one other receptor which is expressed on NK cells in B6/B10 MHC cogenic mice but is difficult to detect in other mouse strains. Redirected lysis experiments indicate that the additional receptor recognized by 14B11 mAb may be an activating receptor since the addition of 14B11 mAb to activated NK cells augmented the lysis of target cells. The mouse homolog of MAFA (mast-cell function-associated antigen) is recognized by 2F1 mAb. MAFA was reported to inhibit the Fcusd\varepsilonusdRI stimulation-induced mast cell secretory response in rats. The function of MAFA on mouse NK cells remains to be determined. Nevertheless, the 2F1 antigen appears to be expressed in an MHC class I-dependent manner. Moreover, the 2F1 antigen is expressed less frequently, and at lower levels by NK cells in MHC class I-deficient mice suggesting an important role for the 2F1 antigen on NK cells.
Subject	:	Biological sciences
	:	Cellular biology
	:	Health and environmental sciences
	:	Immunology
	:	natural killer cells