Fusion proteins containing the starch-binding domains of glucoamylase and cyclodextrin glucanotransf...

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" Fusion proteins containing the starch-binding domains of glucoamylase and cyclodextrin glucanotransferase "
B. K. Dalmia Z. L. Nikolov

Document Type	:	Latin Dissertation
Language of Document	:	English
Record Number	:	1112986
Doc. No	:	TLpq304114778
Main Entry	:	B. K. Dalmia
	:	Z. L. Nikolov
Title & Author	:	Fusion proteins containing the starch-binding domains of glucoamylase and cyclodextrin glucanotransferase\ B. K. DalmiaZ. L. Nikolov
College	:	Iowa State University
Date	:	1994
student score	:	1994
Degree	:	Ph.D.
Page No	:	111
Abstract	:	This work addresses the interactions of a starch-binding domain (SBD) from glucoamylase-I (GA-I) of Aspergillus niger with raw starch and a possible application of the SBD as an affinity tail for a single-step recovery and purification of fusion proteins from crude cell extracts, using native starch granules as an adsorbent. Our previous studies indicated that GA-I adsorbed specifically and strongly to raw corn starch primarily via its SBD, which is located at the C-terminus of the enzyme molecule. A usd\betausd-galactosidase (usd\betausd-gal) fusion protein (BSB119) containing the C-terminal 119 aminoacids from GA-I showed significantly higher affinity for starch than a usd\betausd-gal control. This fusion protein was used as a model system to study the adsorption and elution behavior of SBD from starch. The results showed that the SBD is an independent domain that is functional when attached to another protein and the fusion expressed in E. coli. The adsorbed fusion protein was purified by competitive elution with various oligosaccharides. To test the generality of the proposed fusion protein approach for protein purification, another fusion protein (GSB119) was constructed with the same 119 aminoacids of SBD and glutathione S-transferase. This fusion protein formed inclusion bodies when produced in E. coli. Several procedures to reduce inclusion body formation were examined but were not very successful. The fusion protein was tested for its starch-binding activity and found to bind specifically to starch and easily eluted with 10 mM usd\betausd-cyclodextrin. Cyclodextrin glucanotransferase (CGTase) from Bacillus macerans also contains a starch-binding domain called domain E which is about 60% homologous to the SBD from GA-I. A usd\betausd-gal fusion protein with domain E (BDE109) from CGTase was also constructed and produced in E. coli. The starch-binding activity of BDE109 was compared to that of BSB119. It was found that both the SBD and domain E have similar affinities for the starch granule but different saturation capacities.
Subject	:	Applied sciences
	:	Chemical engineering