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" Evaluation and assessment of the important factors affecting soybean seed germination, quality and storage "
S. Khan
J. G. Mexal
Document Type
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Latin Dissertation
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Language of Document
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English
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Record Number
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1113171
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Doc. No
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TLpq304212651
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Main Entry
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J. G. Mexal
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S. Khan
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Title & Author
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Evaluation and assessment of the important factors affecting soybean seed germination, quality and storage\ S. KhanJ. G. Mexal
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College
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New Mexico State University
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Date
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1995
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student score
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1995
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Degree
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Ph.D.
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Page No
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184
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Abstract
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A series of experiments were conducted to study the effect of temperature, priming and storage on soybean seed quality. In the first study soybeans were planted in the spring (January 13 to March 1) and the fall (July 10 to August 25, 1991) at Agricultural Research Institute Tarnab, Peshawar, Pakistan. The spring planted crop matured during hot weather (May to July), produced heavier seeds, had higher protein, oil, ash content poor germination and more infection with seedborne pathogens. To evaluate the effect of PEG 8000 concentrations and seed treatment durations on germination, experiments were conducted on (a) freshly primed seed and (b) seed stored after priming. In the freshly primed seed study, seed was either primed for 1, 2 or 7 days at osmotic potentials of 0, -0.1, -0.2, -0.4, -0.5, -0.7 and -1.1 MPa, or primed for 1, 2 or 3 days at -0.3, -0.5, -0.9, -1.5 MPa with a dry seed control. Lower water potentials (-1.1, -1.5 MPa) increased germination compared to control. Priming longer than 2 days was deleterious. In seed stored after priming study, two experiments were conducted. In experiment 1, seed from spring and fall crop was primed for 1 or 2 days at osmotic potentials of -0.5, -1.1 and -1.8 MPa). Seed after drying, was stored at ambient and refrigerated temperatures. Seeds were germinated after 12, 16, 22 and 33 weeks storage in laboratory. Seeds harvested from the fall crop had better germination throughout storage period. Both primed and unprimed seed stored under refrigerated conditions showed higher germination throughout storage time. However, unprimed seeds stored at ambient temperature showed a progressive decline in germination over time. In experiment 2, seed from fall crop was primed as in experiment 1 and stored at ambient temperature for 0, 4, 8 and 12 weeks. Seeds were planted in the field at NWFP Agricultural University, Peshawar and Agricultural Research Institute, Tarnab, Pakistan. Priming did not improve germination, shoot weight or plant height, but increased pods per plant and seed yield upto 12 weeks of storage. In the third study, potted soybean were transferred at pod formation stage to three growth chambers fixed at 18/10, 25/15 and 32/20C days and night temperature. Pods were selected from three positions i.e. bottom main stem, mid-main stem and top main stem. One half of the seeds from above pod positions were placed in germination boxes saturated with PEG 8000 (water potential = -0.5 MPa), while second half of the seeds were saturated with distilled water. Seeds were germinated in three chambers fixed at 18, 25 and 35C. Exposure of mother plant to increasing temperature during seed fill stage resulted in a linear decline in seed germination. Seed located at bottom and middle were heavier and has better germination than seed located at top. Germination was highest at 25C and germinated earlier compared with 18 or 35C. PEG stress decreased germination and also delayed germination compared with control.
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Subject
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Agronomy
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Biological sciences
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Glycine max
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PEG 8000
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primed seeds
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seed storage
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