رکورد قبلیرکورد بعدی

" Immunohistochemical analysis of cytochrome P450 isozymes (CYP2B1 & CYP1A1) and NADPH-cytochrome P450 reductase during lung tumour development in SWR/J mice "


Document Type : Latin Dissertation
Language of Document : English
Record Number : 1113217
Doc. No : TLpq304376344
Main Entry : J. A. Lord
: P. G. Forhert
Title & Author : Immunohistochemical analysis of cytochrome P450 isozymes (CYP2B1 CYP1A1) and NADPH-cytochrome P450 reductase during lung tumour development in SWR/J mice\ J. A. LordP. G. Forhert
College : Queen's University (Canada)
Date : 1997
student score : 1997
Degree : M.Sc.
Page No : 86
Abstract : The cytochrome P450 enzymes represent a family of hemoproteins, which together with the flavoprotein NADPH-cytochrome P450 reductase, are responsible for the oxidative metabolism of multiple xenobiotics and endogenous compounds. These enzymes are classified according to proposed evolutionary relationships. As such, an italicized root symbol CYP denotes the cytochrome P450 gene (Cyp for mouse), an Arabic number identifies the family, a letter distinguishes the subfamily and an Arabic numeral denotes the individual gene. Gene products include only capital letters, without italics. Previous studies have postulated that murine lung tumourigenesis may be correlated with decreased cytochrome P450 expression. The present study utilized polyclonal antibodies and avidin-biotin horseradish peroxidase, to immunohistochemically detect CYP2B1, CYP1A1 and NADPH-cytochrome P450 reductase in developing tumours and non-neoplastic tissues within SWR/J murine lung. Mice were administered the procarcinogen urethane (1 mg/g) and sacrificed at designated stages of neoplastic development. Developing tumours were categorized as hyperplastic foci (6-10 weeks), solid or papillary adenomas (16-22 weeks) and solid or papillary carcinomas (52 weeks). To induce CYP1A1, mice were administered usd\betausd-naphthoflavone (usd\betausd-NF), in corn oil (80 mg/kg) prior to sacrifice. Control mice received only the vehicle. In control mice, CYP2B1 was localized in Clara and type II cells, while CYP1A1 was not immunohistochemically detected. Following treatment with usd\betausd-NF, CYP2B1 localization was not altered; however, CYP1A1 was detected in endothelial and type II cells. NADPH-cytochrome P450 reductase was detected in Clara and type II cells of all mice. CYP2B1 was weakly detected in all developing tumours. In usd\betausd-NF-treated mice, CYP1A1 and NADPH-cytochrome P350 reductase were detected in hyperplastic foci, solid adenomas and to a lesser extent in papillary adenomas, but were absent in carcinomas. Furthermore, localization of both CYP1A1 and NADPH-cytochrome P450 reductase was most pronounced in hyperplastic foci within carcinoma-bearing lungs. These results suggest that tumour formation is associated with diminished expression of CYP2B1, CYP1A1, and NADPH-cytochrome P450 reductase.
Subject : Biological sciences
: Cellular biology
: Cellular biology
: Health and environmental sciences
: Molecular biology
: Oncology
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