Genetic variation in strains of Clavibacter michiganensis subsp. michiganensis and the development o...

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" Genetic variation in strains of Clavibacter michiganensis subsp. michiganensis and the development of bird's eye fruit lesions on tomatoes "
C. M. Medina-Mora

Document Type	:	Latin Dissertation
Language of Document	:	English
Record Number	:	1113255
Doc. No	:	TLpq304522779
Main Entry	:	C. M. Medina-Mora
Title & Author	:	Genetic variation in strains of Clavibacter michiganensis subsp. michiganensis and the development of bird's eye fruit lesions on tomatoes\ C. M. Medina-Mora
College	:	Michigan State University
Date	:	1999
student score	:	1999
Degree	:	M.S.
Page No	:	145
Abstract	:	Bacterial canker of tomato is a major concern worldwide because disease occurrence is sporadic and may result in large yield reductions. Computer-assisted analysis of repetitive sequence-based Polymerase Chain Reaction (rep-PCR) genomic fingerprints generated with the universal primers BOX and (GTG)5 suggested genomic variation in Clavibacter michiganensis subsp. michiganensis (Cmm) is relatively limited. However, (GTG)5-PCR fingerprint patterns do differentiate strains within the previously designated BOX-PCR types (A, B, C, D, and E). Intraspecific diversity using combined (GTG)5-PCR and BOX-PCR data is as follows; usd\rm C>A\ge B>D>Eusd. These data may provide useful genetic markers to trace Cmm strains used in epidemiological studies. Fruit spotting resulting from Cmm infection is one of the most unpredictable tomato disease symptoms of bacterial canker in Michigan. Therefore, fruit spot formation was studied by spraying flowers with Cmm at various developmental stages. Maximum incidence of spotted fruit and maximum severity of fruit lesions resulted when inoculum was applied twice, a possible indication that a bacterial population threshold is required for the onset of fruit spots. Chemical control strategies could be formulated to reduce the level of unmarketable tomato fruit.
Subject	:	Biological sciences
	:	Genetics
	:	Plant pathology