This page uses JavaScript and requires a JavaScript enabled browser.Your browser is not JavaScript enabled.
مرکز و کتابخانه مطالعات اسلامی به زبان های اروپایی
منو
درگاههای جستجو
مدارک
جستجوی پیشرفته
مرور
جستجو در سایر کتابخانه ها
مستندات
جستجوی پیشرفته
مرور
منابع دیجیتال
تمام متن
اصطلاحنامه
درختواره
پرسش و پاسخ
سوالات متداول
پرسش از کتابدار
پیگیری پرسش
ورود
ثبت نام
راهنما
خطا
رکورد قبلی
رکورد بعدی
"
DNA-protein cross-linking by bis-electrophiles
"
Rachel Lea Loeber
N. Tretyakova
Document Type
:
Latin Dissertation
Language of Document
:
English
Record Number
:
52449
Doc. No
:
TL22403
Call number
:
3330512
Main Entry
:
Rachel Lea Loeber
Title & Author
:
DNA-protein cross-linking by bis-electrophiles\ Rachel Lea Loeber
College
:
University of Minnesota
Date
:
2008
Degree
:
Ph.D.
student score
:
2008
Page No
:
255
Abstract
:
The genotoxic effects of bis -electrophiles are generally attributed to their ability to cross-link biomolecules. While DNA-DNA cross-linking mediated by 1,2,3,4-diepoxybutane (DEB) and antitumor nitrogen mustards is well characterized and thought to be largely responsible for their cytotoxic effects, the association between DNA-protein cross-linking and genotoxicity is less clear. Acting as bulky, helix-distorting lesions, DNA-protein cross-links block binding and progression of protein complexes, potentially interfering with crucial cellular processes. In the current work, the ability of DEB and nitrogen mustards to induce DNA-protein cross-links was examined in vitro. DEB- and nitrogen mustard-induced cross-linking was first established between double-stranded oligodeoxynucleotides and the human DNA repair protein O6 -alkylguanine DNA alkyltransferase (AGT). Cross-link formation was confirmed by SDS-PAGE, and tandem mass spectrometry was employed to elucidate cross-link structures. Next, the ability of DEB and nitrogen mustards to cross-link AGT to DNA in the presence of other cellular proteins was examined via Western blot analysis and tandem mass spectrometry. The effect of AGT expression in DEB- and nitrogen mustard-induced cytotoxicity and mutagenicity was then investigated in Chinese hamster ovary cells expressing the human protein. Lastly, additional proteins that had become cross-linked to DNA in the presence of the nitrogen mustard mechlorethamine were identified using a combination of Western blot analysis and shot-gun proteomics. As we have demonstrated that DNA-protein cross-links form upon exposure to DEB and nitrogen mustards, cross-links of this type are likely to contribute to the cytotoxicity of bis -electrophiles in mammalian cells.
Subject
:
Health and environmental sciences; Pure sciences; Bis-electrophiles; Cross-linking; Mass spectrometry; Nitrogen mustards; Toxicology; Analytical chemistry; Biochemistry; 0486:Analytical chemistry; 0383:Toxicology; 0487:Biochemistry
Added Entry
:
N. Tretyakova
Added Entry
:
University of Minnesota
https://lib.clisel.com/site/catalogue/52449
کپی لینک
پیشنهاد خرید
پیوستها
عنوان :
نام فایل :
نوع عام محتوا :
نوع ماده :
فرمت :
سایز :
عرض :
طول :
3330512_8556.pdf
3330512.pdf
پایان نامه لاتین
متن
application/octet-stream
8.20 MB
85
85
نمایش
نظرسنجی
نظرسنجی منابع دیجیتال
1 - آیا از کیفیت منابع دیجیتال راضی هستید؟
X
کم
متوسط
زیاد
ذخیره
پاک کن