Multilocus sequence typing of historical Burkholderia pseudomallei isolates

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" Multilocus sequence typing of historical Burkholderia pseudomallei isolates "
Roberta Lea McCombie

Document Type	:	Latin Dissertation
Language of Document	:	English
Record Number	:	52776
Doc. No	:	TL22730
Call number	:	‭MR34235‬
Main Entry	:	Roberta Lea McCombie
Title & Author	:	Multilocus sequence typing of historical Burkholderia pseudomallei isolates\ Roberta Lea McCombie
College	:	University of Calgary (Canada)
Date	:	2007
Degree	:	M.Sc.
student score	:	2007
Page No	:	123
Abstract	:	A collection of 207 historically relevant Burkholderia pseudomallei isolates was analyzed by multilocus sequence typing. The collection contains environmental isolates from a geographical survey of B. pseudomallei in Thailand during 1964 to 1967, clinical isolates from American soldiers serving in Vietnam, and stock cultures and colony variants from the United States Army Medical Research Unit (Malaysia), the Walter Reed Army Institute for Research, and the Pasteur Institute (Vietnam). The collection was resolved into 80 sequence types, 56 were novel. Differences in colony morphology among reference isolates did not affect ST assignment and the first environmental isolates sharing ST10 with B. pseudomallei K96243 were reported. eBURST analysis of the historical collection demonstrated an ancestral nature of the isolates when compared with all isolates catalogued in the B. pseudomallei MLST database. Australian ST84 was found to be characteristic of B. pseudomallei isolates from Southeast Asia and an epidemiologically significant phylogenetic relationship was discovered between Thai and Australian environmental isolates through the commonality of ST60, which has important implications for future studies in melioidosis epidemiology.
Subject	:	Biological sciences; Microbiology; 0410:Microbiology
Added Entry	:	University of Calgary (Canada)