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" Discovery of pro-migratory, cancer associated genes "
Abu Amara Suha Naffar
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Latin Dissertation
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English
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| Record Number
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53271
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TL23225
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3490026
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| Main Entry
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Abu Amara Suha Naffar
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| Title & Author
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Discovery of pro-migratory, cancer associated genes\ Abu Amara Suha Naffar
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| College
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The Weizmann Institute of Science (Israel)
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| Date
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2008
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| Degree
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Ph.D.
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2008
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123
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| Abstract
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The primary objective of this research was to identify and characterize tumor-related genes and transcriptional or signaling modulators that affect cell adhesion and motility, and to characterize their mode(s) of activity. Identification of pro-migratory genes is critical to our ability to understand cancer cell migration and, as a consequence, the metastatic process. In order to test the effect of many genes on the migration of cell populations, our first aim was to develop a two-dimensional high-throughput screening system that is reproducible, capable of indicating alterations in migratory behavior in both the overall population of treated cells, as well as in individual cells within this population, in order to enable the quantitative analysis of cell motility. Our next aim was to search for pro-migratory and adhesion-related genes within two collections of genes. The first collection was composed of cDNA clones from MDA-MB231 human breast carcinoma, a highly migratory and metastatic cell line. The second collection was taken from the BC100 library, and included genes that are known to be involved in cancer progression; however, their precise roles in cancer cell migration have not yet been clarified. Next, we worked to identify and characterize the effects of the promigratory genes on specific components of the migratory process (persistence, velocity, and the like), and to characterize the cellular changes induced by these genes on the cytoskeleton and on cell-matrix adhesions. Finally, we tested the effects of selected pro-migratory genes on tumor invasiveness. AIl of the aforementioned aims will be discussed in greater depth in the following chapters.
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Biological sciences; Cell migration; Extracellular matrix; Molecular biology; 0307:Molecular biology
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P. enter
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The Weizmann Institute of Science (Israel)
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