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Document Type:Latin Dissertation
Language of Document:English
Record Number:53723
Doc. No:TL23677
Call number:‭MR52661‬
Main Entry:Nazia Islam Pathan
Title & Author:Ultrastructural examination of Arabidopsis thaliana wild type and ahp2 mutant meiotic nuclei using serial sections for transmission electron microscopyNazia Islam Pathan
College:University of Toronto (Canada)
Date:2009
Degree:M.Sc.
student score:2009
Page No:119
Abstract:The progressive changes in the chromosome alignment processes in wild type and ahp2 mutant of Arabidopsis thaliana (Ler ) have been studied using the serial ultrathin-sectioning technique for transmission electron microscopy. This technique preserves weak alignments between chromosome axes as well as synaptonemal complexes (SC), and has allowed identification of at least two other structures previously not described in Arabidopsis meiocytes. The ahp2 mutant has severely reduced SC, resulting in only 5.1% SC, compared to 84% in wild type. It is clear that the ahp2 mutant can undergo rough alignment (44.0% of total bivalent length) and does convert appreciable amounts of the rough alignment into close alignments (39.0% of the total bivalent length). Thus, we have proposed that the Ahp2 mediated strand invasion may be required once rough alignment occurs and may facilitate close alignment. Lack of Ahp2 may delay close alignment and prevent appreciable amounts of SC.
Subject:Biological sciences; Genetics; Cellular biology; 0369:Genetics; 0379:Cellular biology
Added Entry:University of Toronto (Canada)