| | | Document Type | : | Latin Dissertation | | Language of Document | : | English | | Record Number | : | 55222 | | Doc. No | : | TL25176 | | Call number | : | 1472148 | | Main Entry | : | Marielis Torres-Medina | | Title & Author | : | Probiotic-supplemented soy bar effects on resistance to infection by Listeria monocytogenesMarielis Torres-Medina | | College | : | University of Missouri - Columbia | | Date | : | 2008 | | Degree | : | M.S. | | student score | : | 2008 | | Page No | : | 108 | | Abstract | : | Probiotics are living microorganisms that help regulate the gastrointestinal tract. The aim of this project was to investigate the benefits of probiotics and their inhibitory effect on Listeria monocytogenes EGD. The well diffusion assay was conducted to screen the probiotics for inhibition of L. monocytogenes EGD. A diet was created out of a soy protein bar supplemented with the probiotics to be used in our in vivo test using a mouse model. Three groups of mice were fed for two weeks with three different diets, one diet each, Control diet with no probiotic, ADH diet with Lactobacillus acidophilus ADH, and B6 diet with Bifidobacterium animalis B6. Each group was subdivided into two different groups, one of them would be challenged with L. monocytogenes EGD and the other group would not (control group). After 14 days of feeding, the mice were challenged intragastrically with ~108 CFU/ml L. monocytogenes EGD. At day 3 post-infection, the mice were euthanized. Of the samples collected, the colon and cecum were tested for probiotic concentration. The spleen and liver were tested for the presence of L. monocytogenes . In the first replication, a half a log reduction of L. monocytogenes EGD in the liver was observed in the Bifidobacterium group as compared with the control group, but no significant reduction in the pathogen was seen in the Lactobacillus group for any of the samples collected. For the second trial, a one log reduction of L. monocytogenes EGD in the liver was achieved in the Bifidobacterium group, but at the same time, a one log reduction of the pathogen in the spleen was also observed in the Lactobacillus group. We also collected evidence showing that the probiotics colonized the colon and the cecum with concentrations of ~107 CFU. According to our results we believe that the probiotic supplemented soy protein bar holds promise to prevent listeriosis. | | Subject | : | Biological sciences; Agronomy; Microbiology; Animal sciences; 0359:Agronomy; 0475:Animal sciences; 0410:Microbiology | | Added Entry | : | A. Mustapha | | Added Entry | : | University of Missouri - Columbia |
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