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" High-Performance Liquid Chromatography of Peptides and Proteins : "
editors, Hodges, Robert S.
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BL
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554944
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b383517
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Colin T Mant
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High-Performance Liquid Chromatography of Peptides and Proteins : : Separation, Analysis, and Conformation\ editors, Hodges, Robert S.
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| Edition Statement
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First edition
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| Publication Statement
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Boca Raton, FL : CRC Press,, 2017.
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text file, PDF
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| ISBN
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0203751949
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: 1351440748
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: 1351440756
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: 9780203751947
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: 9781351440745
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: 9781351440752
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| Contents
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Cover Title Page Copyright Page Preface Table of Contents SECTION I: PRACTICAL ASPECTS OF HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY Properties of Peptides/Proteins and Practical Implications Standard Chromatographic Conditions for Size-Exclusion, Ion-Exchange, Reversed-Phase and Hydrophobic Interaction Chromatography Preventive Maintenance and Troubleshooting LC Instrumentation Tools and Techniques to Extend LC Column Lifetimes Mobile Phase Preparation and Column Maintenance HPLC Guard Columns On-Line Derivatization of Silica Supports for Regeneration of Reversed-Phase Columns. SECTION II: THEORETICAL ASPECTS OF HIGH-PERFORMANCE LIQUID CHROMATOGRAPHYHPLC Terminology: Practical and Theoretical High-Performance Liquid Chromatography of Peptides and Proteins: General Principles and Basic Theory High-Performance Liquid Chromatography of Peptides and Proteins: Quantitative Relationships for Isocratic and Gradient Elution of Peptides and Proteins SECTION III: SIZE-EXCLUSION CHROMATOGRAPHY Requirement for Peptide Standards to Monitor Ideal and Non-Ideal Behavior in Size- Exclusion Chromatography High-Perfonnance Size-Exclusion Chromatography of Proteins. Size-Exclusion Chromatography of ProteinsSize-Exclusion High-Performance Liquid Chromatography of Integral Membrane Proteins and the Effect of Detergents on Immunological Activity A Reversed-Phase Trace Enrichment Technique for the Loading of Size-Exclusion HPLC Columns SECTION IV: ION-EXCHANGE CHROMATOGRAPHY The Use of Peptide Standards for Monitoring Ideal and Non-Ideal Behavior in Cation-Exchange Chromatography Ion-Exchange High-Performance Liquid Chromatography of Peptides Use of Ion-Exchange Cartridges for Batch Extraction in the Purification of Pituitary Peptides. High-Performance Ion-Exchange Chromatography of ProteinsHigh-Performance Ion-Exchange Chromatography of Proteins: Application to the Purification of Enzymes Anion Exchange High-Performance Liquid Chromatography of Viral Proteins Sample Preparation and Application of Different HPLC Methods for the Separation of Hydrophobic Membrane Proteins Cation-Exchange High-Performance Liquid Chromatography: Group Separation of Acidic and Basic Proteins Using Volatile Solvents Separation of Protein Isoforms: An Ion-Exchange HPLC Separation of Bovine and Cardiac Troponin Complexes. Assessment of Steroid Receptor Polymorphism by High-Performance Ion-Exchange ChromatographySECTION V: REVERSED-PHASE CHROMATOGRAPHY Reversed-Phase Packings for the Separation of Peptides and Proteins by Means of Gradient Elution High-Performance Liquid Chromatography Commercially Available Columns and Packings for Reversed-Phase HPLC of Peptides and Proteins Requirement for Peptide Standards to Monitor Colunm Performance and the Effect of Column Dimensions, Organic Modifiers, and Temperature in Reversed-Phase Chromatography.
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Electronic books
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Robert S Hodges
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