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" Stable isotope labeling by amino acids in cell culture (SILAC) "
edited by Bettina Warscheid, Institute of Biology II, University of Freiburg, Germany.
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BL
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| Record Number
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569087
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| Doc. No
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b398306
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| Title & Author
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Stable isotope labeling by amino acids in cell culture (SILAC) : methods and protocols /\ edited by Bettina Warscheid, Institute of Biology II, University of Freiburg, Germany.
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| Publication Statement
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New York, NY :: Humana Press,, [2014]
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| Series Statement
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Methods in Molecular Biology,; 1188
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| ISBN
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9781493911417
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: 1493911414
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: 9781493911431
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9781493911424
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| Bibliographies/Indexes
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Includes bibliographical references and index.
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| Abstract
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"Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC): Methods and Protocols provides a synopsis of a large array of different SILAC methods by presenting a set of protocols that have been established by renowned scientists and their working groups.These include methods and protocols for the labeling of various model organisms as well as advanced strategies relying on SILAC, e.g. for the analysis of protein interactions, the mapping of posttranslational modifications or the characterization of subcellular proteomes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-bystep, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls."--
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| Subject
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Proteomics, Laboratory manuals.
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Stable isotopes, Laboratory manuals.
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Amino acids, Laboratory manuals.
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Cell culture, Laboratory manuals.
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| Subject
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Mass spectrometry.
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| Added Entry
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Warscheid, Bettina.
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