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" Glycosyltransferases "
edited by Inka Brockhausen.
Document Type
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BL
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Record Number
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569614
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Doc. No
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b398833
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Title & Author
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Glycosyltransferases : methods and protocols /\ edited by Inka Brockhausen.
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Publication Statement
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New York :: Humana Press,, [2013]
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Series Statement
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Springer protocols
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Methods in molecular biology,; 1022
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ISBN
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9781627034647
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: 1627034641
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9781627034654
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Bibliographies/Indexes
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Includes bibliographical references and index.
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Contents
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Glycan microarray screening assay for glycosyltransferase specificities / W. Peng, C.M. Nycholat and N. Razi -- A fluorescence-based assay for core 1 [beta]3galactosyltransferase (T-synthase) activity / T. Ju and R.D. Cummings -- Structural and biochemical analysis of a bacterial glycosyltransferase / F. Zhu ... [et al.] -- Study of the biological functions of mucin type core 3 O-glycans / S.H. Lee and M. Fukuda -- Generation of anti-sulfated glycan antibodies using sulfotransferase-deficient mice / H. Kawashima -- Fluorescent microscopy as a tool to elucidate dysfunction and mislocalization of golgi glycosyltransferases in COG complex depleted mammalian cells / R.A. Willett, I.D. Pokrovskaya and V.V. Lupashin -- A practical approach to reconstruct evolutionary history of animal sialyltransferases and gain insights into the sequence-function relationships of golgi-glycosyltransferases / D. Petit ... [et al.] -- Fluorescent lectin staining of drosophila embryos and tissues to detect the spatial distribution of glycans during development / E. Tian, L. Zhang and K.G. Ten Hagen -- Photoaffinity labeling of protein O-mannosyltransferases of the PMT1/PMT2 subfamily / M. Loibl and S. Strahl -- Enzymatic analysis of the protein O-glycosyltransferase, rumi, acting toward epidermal growth factor-like (egf) repeats / H. Takeuchi and R.S. Haltiwanger -- Enzymatic characterization of recombinant enzymes of O-glcNAc cycling / E.J. Kim and J.A. Hanover -- Antibodies and activity measurements for the detection of o-glcnac transferase and assay of its substrate, UDP-glcNAc / T. Lefebvre ... [et al.] -- In vitro glycosylation assay for bacterial oligosaccharyltransferases / M.A. Musumeci, M.V. Ielmini and M.F. Feldman -- In vitro UDP-sugar:Undecaprenyl-phosphate sugar-1-phosphate transferase assay and product detection by thin layer chromatography / K.B. Patel and M.A. Valvano -- In vitro O-antigen ligase assay / X. Ruan and M.A. Valvano -- Functional identification of bacterial glucosyltransferase WbdN / Y. Gao, A. Vinnikova and I. Brockhausen -- Methods for the pasteurella glycosaminoglycan synthases: Enzymes that polymerize hyaluronan, chondroitin, or heparosan chains / P.L. Deangelis -- Methods for measuring class i membrane-bound hyaluronan synthase activity / P.H. Weigel, A.J. Padgett-McCue and B.A. Baggenstoss -- Creation and characterization of glycosyltransferase mutants of Trypanosoma brucei / L. Izquierdo, M.L. Guther and M.A. Ferguson -- Mannose-6-phosphate: A regulator of LLO destruction / N. Gao and M.A. Lehrman -- N-acetylglucosaminyltransferase (GnT) assays using fluorescent oligosaccharide acceptor substrates: GnT-III, IV, V, and IX (GnT-Vb) / S. Takamatsu ... [et al.] -- A method for determination of UDP-GlcNAc: GlcNAc[beta]1-6(GlcNAc[beta]1-2)man[alpha]1-r [GlcNAc to man] beta1-4n-acetylglucosaminyltransferase vi activity / T. Taguchi and N. Taniguchi -- In vitro assays of orphan glycosyltransferases and their application to identify notch xylosyltransferases / M.K. Sethi ... [et al.] -- In vitro folding of [beta]-1,4galactosyltransferase and polypeptide-[alpha]-N-acetylgalactosaminyltransferase from the inclusion bodies / B. Ramakrishnan and P.K. Qasba -- An assay for [alpha] 1,6-fucosyltransferase (FUT8) activity based on the HPLC separation of a reaction product with fluorescence detection / H. Ihara ... [et al.] -- Immunodetection of glycosyltransferases in gastrointestinal tissues / J. Gomes and C.A. Reis -- Glycosyltransferases in chemo-enzymatic synthesis of oligosaccharides / B. Tefsen and I. van Die -- Tumor targeting by a carbohydrate ligand-mimicking peptide / S. Hatakeyama ... [et al.] -- Glycoengineering of human cell lines using zinc finger nuclease gene targeting: Simple cells with homogeneous GalNAc O-glycosylation allow isolation of the O-glycoproteome by one-step lectin affinity chromatography / C. Steentoft, E.P. Bennett and H. Clausen -- Generation of monoclonal antibodies to native active human glycosyltransferases / M.B. Vester-Christensen ... [et al.].
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Subject
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Glycosyltransferases, Laboratory manuals.
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Subject
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Glycosyltransferases, Laboratory Manuals.
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Subject
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Glycosylation, Laboratory Manuals.
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Added Entry
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Brockhausen, Inka,1944-
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Cover Title
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Glycosyl-transferases
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