|
" DNA replication "
edited by Sonya Vengrova, Coventry, UK, Jacob Dalgaard, Warwick Medical School, University of Warwick, Coventry, UK.
| Document Type
|
:
|
BL
|
| Record Number
|
:
|
575854
|
| Doc. No
|
:
|
b405073
|
| Uniform Title
|
:
|
DNA replication (Vengrova)
|
| Title & Author
|
:
|
DNA replication : methods and protocols /\ edited by Sonya Vengrova, Coventry, UK, Jacob Dalgaard, Warwick Medical School, University of Warwick, Coventry, UK.
|
| Edition Statement
|
:
|
Second edition.
|
| Publication Statement
|
:
|
New York :: Humana Press,, [2015]
|
| Series Statement
|
:
|
Methods in molecular biology,; 1300
|
|
|
:
|
Springer protocols
|
| ISBN
|
:
|
9781493925957
|
|
|
:
|
: 1493925954
|
|
|
:
|
9781493925964
|
|
|
:
|
1493925962
|
| Bibliographies/Indexes
|
:
|
Includes bibliographical references and index.
|
| Contents
|
:
|
A High-Throughput Confocal Fluorescence Microscopy Platform to Study DNA Replication Stress in Yeast Cells -- Microscopy Techniques to Examine DNA Replication in Fission Yeast -- High-Resolution Analysis of Mammalian DNA Replication Units -- Analyzing the Dynamics of DNA Replication In Mammalian Cells Using DNA Combing -- Measuring DNA Content by Flow Cytometry in Fission Yeast -- Incorporation of Thymidine Analogs for Studying Replication Kinetics in Fission Yeast -- EdU Incorporation for FACS and Microscopy Analysis of DNA Replication in Budding Yeast -- Determination of Deoxyribonucleoside Triphosphate Concentrations in Yeast Cells by Strong Anion-Exchange High-Performance Liquid Chromatography Coupled with Ultraviolet Detection -- Measuring Ribonucleotide Incorporation Into DNA In Vitro and In Vivo -- Detection and Sequencing of Okazaki Fragments In S. cerevisiae -- ChIP-Seq to Analyze the Binding of Replication Proteins to Chromatin -- Chromatin Immunoprecipitation to Detect DNA Replication and Repair Factors -- Molecular Genetic Methods to Study DNA Replication Protein Function in Haloferax volcanii, A Model Archaeal Organism -- Single-Molecule Observation of Prokaryotic DNA Replication -- Analyzing the Response to Dysfunction Replication Forks Using the RTS1 Barrier System in Fission Yeast -- Purification of Restriction Fragments Containing Replication Intermediates from Complex Genomes for 2-D Gel Analysis -- Isolation of Restriction Fragments Containing Origins of Replication from Complex Genomes.
|
| Abstract
|
:
|
Updated and revised, this thorough volume covers a range of methods focusing on systems, including mammalian, yeast, bacterial and archaeal. This second edition of DNA Replication: Methods and Protocols describes approaches to analyze whole genomes to single molecules, as well as both in vivo and in vitro experiments. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, DNA Replication: Methods and Protocols, Second Edition provides a collections of methods intended for newcomers to this research field and for established laboratories.
|
| Subject
|
:
|
DNA replication-- Research-- Methodology.
|
| Subject
|
:
|
DNA replication, Laboratory manuals.
|
| Subject
|
:
|
DNA Replication.
|
| Added Entry
|
:
|
Vengrova, Sonya.
|
|
|
:
|
Dalgaard, Jacob Z.
|
| |