| Document Type
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BL
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| Record Number
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576299
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| Doc. No
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b405518
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| Title & Author
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Chemical proteomics : : methods and protocols /\ edited by Gerard Drews and Marcus Bantscheff.
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| Publication Statement
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New York :: Humana Press,, c2012.
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| Series Statement
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Methods in Molecular Biology ;; 803
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| Page. NO
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xi, 313 p. :: ill. ;; 27 cm.
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| ISBN
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9781617793639
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: 1617793639
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| Bibliographies/Indexes
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Includes bibliographical references and index.
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| Contents
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Mass spectrometry-based chemoproteomic approaches -- Chemical proteomics in drug discovery -- Compound immobilization and drug-affinity chromatography -- Affinity-based chemoproteomics with small molecule-peptide conjugates -- A chemical proteomic probe for detecting dehydrogenases: Catechol rhodanine -- Probing proteomes with benzophenone photoprobes -- Biotinylated probes for the analysis of protein modification by electrophiles -- Profiling of methyltransferases and other S-adenosyl-l: -homocysteine-binding proteins by capture compound mass spectrometry -- Identifying cellular targets of small-molecule probes and drugs with biochemical enrichment and SILAC -- Determination of kinase inhibitor potencies in cell extracts by competition binding assays and isobaric mass tags -- Affinity-based profiling of dehydrogenase subproteomes -- Probing the specificity of protein-protein interactions by quantitative chemical proteomics -- Fluorescence-based proteasome activity profiling -- Chemical cross-linking and high-resolution mass spectrometry to study protein-drug interactions -- Monitoring ligand modulation of protein-protein interactions by chemical cross-linking and high-mass MALDI mass spectrometry -- Time-controlled transcardiac perfusion crosslinking for in vivo interactome studies -- Ligand discovery using small-molecule microarrays -- Working with small molecules : preparing and storing stock solutions and determination of kinetic solubility -- A database for chemical proteomics : ChEBI -- Working with small molecules: Rules-of-thumb of Drug likeness.
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| Subject
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Proteomics, Laboratory manuals.
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| Subject
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Biochemistry, Laboratory manuals.
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| Added Entry
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Drewes, Gerard.
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Bantscheff, Marcus.
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