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" Vaccinia virus and poxvirology : "
edited by Stuart N. Isaacs.
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BL
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| Record Number
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576318
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b405537
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| Title & Author
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Vaccinia virus and poxvirology : : methods and protocols /\ edited by Stuart N. Isaacs.
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| Edition Statement
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2nd ed.
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| Publication Statement
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New York :: Humana Press,, 2012.
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| Series Statement
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Methods in molecular biology ;; 890
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| Page. NO
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xiv, 332 p. :: ill. ;; 26 cm.
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| ISBN
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1617798754
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: 9781617798757
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| Notes
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"Nine years ago, the chapters for the first edition of Vaccinia Virus and Poxvirology were submitted for publication in the Methods in Molecular Biology Series. This second edition does not replace the first edition since essentially every chapter in this volume represents a protocol not covered in the first edition." - p. vii
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| Bibliographies/Indexes
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Includes bibliographical references and index.
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| Contents
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1. Working safely with vaccinia virus: laboratory technique and review of published cases of accidental laboratory infections / Stuart N. Isaacs -- 2. In-Fusion® cloning with vaccinia virus DNA polymerase / Chad R. Irwin, Andrew Farmer, David O. Willer, and David H. Evans -- 3. Genetic manipulation of poxviruses using bacterial artificial chromosome recombineering / Matthew G. Cottingham -- 4. Easy and efficient protocols for working with recombinant Vaccinia virus MVA / Melanie Kremer, Asisa Volz, Joost H.C.M. Kreijtz, Robert Fux, Michael H. Lehmann, and Gerd Sutter -- 5. Isolation of recombinant MVA using F13L selection / Juana M. Sánchez-Puig, María M. Lorenzo, and Rafael Blasco -- 6. Screening for vaccinia virus egress inhibitors: separation of IMV, IEV, and EEV / Chelsea M. Byrd and Dennis E. Hruby -- 7. Imaging of vaccinia virus entry into HeLa cells / Cheng-Yen Huang and Wen Chang -- 8. New method for the assessment of Molluscum contagiosum virus infectivity / Subuhi Sherwani, Niamh Blythe, Laura Farleigh, and Joachim J. Bugert -- 9. An Intradermal model for vaccinia virus pathogenesis in mice / Leon C.W. Lin, Stewart A. Smith, and David C. Tscharke -- 10. Measurements of vaccinia virus dissemination using whole body imaging: approaches for predicting of lethality in challenge models and testing of vaccines and antiviral treatments / Marina Zaitseva, Senta Kapnick, and Hana Golding -- 11. Mousepox, a small animal model of smallpox / David Esteban, Scott Parker, Jill Schriewer, Hollyce Hartzler,and R. Mark Buller -- 12. Analyzing CD8 T cells in mouse models of poxvirus infection / Inge E.A. Flesch, Yik Chun Wong, and David C. Tscharke -- 13. Generation and characterization of monoclonal antibodies specific for vaccinia virus / Xiangzhi Meng and Yan Xiang -- 14. Bioinformatics for analysis of poxvirus genomes / Melissa Da Silva and Chris Upton -- 15. Antigen presentation assays to investigate uncharacterized immunoregulatory genes / Rachel L. Roper -- 16. Characterization of poxvirus-encoded proteins that regulate innate immune signaling pathways / Florentina Rus, Kayla Morlock, Neal Silverman, Ngoc Pham, Girish J. Kotwal, and William L. Marshall -- 17. Application of quartz crystal microbalance with dissipation monitoring technology for studying interactions of poxviral proteins with their ligands / Amod P. Kulkarni, Lauriston A. Kellaway, and Girish J. Kotwal -- 18. Central nervous system distribution of the poxviral proteins after intranasal administration of proteins and titering of vaccinia virus in the brain after intracranial administration / Amod P. Kulkarni, Dhirendra Govender, Lauriston A. Kellaway, and Girish J. Kotwal
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| Subject
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Vaccinia, Laboratory manuals.
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Recombinant poxviruses, Laboratory manuals.
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DNA Replication.
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Molecular Biology-- methods.
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Poxviridae-- genetics.
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Vaccinia virus-- genetics.
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Vaccinia virus-- immunology.
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Virus Replication.
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| Added Entry
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Isaacs, Stuart Neal.
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