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" Lymphocyte Hybridomas "
edited by F. Melchers, M. Potter, N. L. Warner.
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BL
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577335
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b406554
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| Main Entry
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Melchers, F.
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Lymphocyte Hybridomas : Second Workshop on 'Functional Properties of Tumors of T and B Lymphocytes' /\ edited by F. Melchers, M. Potter, N. L. Warner.
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| Publication Statement
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Berlin, Heidelberg :: Springer Berlin Heidelberg,, 1979.
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| ISBN
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9783642674488
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: 9783540096702
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| Contents
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Fusion of Mouse Myeloma and Spleen Cells -- Anti-Viral and Anti-Tumor Antibodies Produced by Somatic Cell Hybrids -- Analysis of V Gene Expression in the Immune Response by Cell Fusion -- Production of Hybrid Lines Secreting Monoclonal Anti-Idiotypic Antibodies by Cell Fusion on Membrane Filters -- A Hybridoma Secreting IgM Anti-Iglb Allotype -- Monoclonal Antibodies Against Developing Chick Brain and Muscle -- Monoclonal Xenogeneic Antibodies to Mouse Leukocyte Antigens: Identification of Macrophage-Specific and Other Differentiation Antigens -- Monoclonal Antibody Detecting a Stage-Specific Embryonic Antigen (SSEA-1) on Preimplantation Mouse Embryos and Teratocarcinoma Cells -- Monoclonal Anti-Rat MHC (H-1) Alloantibodies -- Selective Suppression of Reactivity to Rat Histocompatibility Antigens by Hybridoma Antibodies -- Monoclonal Antibodies to Human Lymphocyte Membrane Antigens -- Expression of Murine IgM, IgD and Ia Molecules on Hybrids of Murine LPS Blasts with a Syrian Hamster B Lymphoma -- Hybrid Plasmacytoma Production: Fusions with Adult Spleen Cells, Monoclonal Spleen Fragments, Neonatal Spleen Cells and Human Spleen Cells -- Fusion of in vitro Immunized Lymphoid Cells with X63Ag8 -- Monoclonal Antibodies Against Murine Cell Surface Antigens: Anti-H-2, Anti-Ia and Anti-T Cell Antibodies -- Mouse Myeloma - Spleen Cell Hybrids: Enhanced Hybridization Frequencies and Rapid Screening Procedures -- Murine Anti-? (1?3) Dextran Antibody Production by Hybrid Cells -- Properties of Monoclonal Antibodies to Mouse Ig Allotypes, H-2, and Ia Antigens -- The Antibody Repertoire of Hybrid Cell Lines Obtained by Fusion of X63-AG8 Myeloma Cells with Mitogen-Activated B-Cell Blasts -- Mouse ?1 Hybridomas -- Rapid Binding Test for Detection of Alloantibodies to Lymphocyte Surface Antigens -- Cellular and Molecular Restrictions of the Lymphocyte Fusion -- Production of Specific Antibody Without Specific Immunization -- Clones of Human Lymphoblastoid Cell Lines Producing Antibody to Tetanus Toxoid -- Establishment of Specific Antibody Producing Human Lines by Antigen Preselection and EBV-Transformation -- Human Normal and Leukemia Cell Surface Antigens. Mouse Monoclonal Antibodies as Probes -- Mouse-Human Hybridomas. The Conversion of Non-Secreting Human B Cells into Ig Secretors -- Induction of IgM Secretion by Fusing Murine B-Lymphoma with Myeloma Cells -- Cloned T-Cell Lines with Specific Cytolytic Activity -- Characterization and Functional Analysis of T Cell Hybrids -- Expression of Thy 1.2 Antigen on Hybrids of B Cells and a T Lymphoma -- T Cell Hybrids with T Cell Functions -- T Cell Hybrids with Specificity for Individual Antigens -- Specific Suppressor T Cell Hybridomas -- Selective Expression of Loci in the I-J Region on T Cell Hybrids -- Attempts to Produce Cytolytically Active Cell Hybrids Using EL4 and MLC Spleen -- Phenotypic Expression in T-Lymphocyte x 'Fibroblast' Cell Hybrids -- A Quantitative Disc Radioimmunoassay for Antibodies Directed Against Membrane-Associated Antigens -- Summary of Cloning of Differentiated Function Using Hybrid Cells and Comparison of the Mouse and Human Gene Maps for Homologous Markers -- List of Contributors.
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| Abstract
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F. Melchers, M. Potter, NL. Warner HISTORICAL Prior to 1964 many workers had experienced considerable difficulty in establishing continuous lines of murine plasmacytoma in culture. Pettingill and Sorenson in 1966 grew X5563 after a painstaking period of adaptation. During a visit to the Salk Instiute in 1965 Leo Sachs began experiments with K. Horibata,E. S. Lennox and M. Cohn that ulti mateley convinced most workers that murine plasmacytomas could be grown in vitro with ease. They adapted MOPC-21 * {called P3 at the Salk Institute) to in vitro culture and showed the cells produced a large amount of the IgG1 myeloma protein. Horibata, Lennox and Sachs did not report the important finding though a manuscript was written and accepted. The reason for the failure to publish the paper was that the original line was not recovered from the freezer and knowing there would be many requests for it the paper was withdrawn. As it later turned out, the failure to recover the line may have been triv ial, for Horibata and Harris (1970) soon re-established a continuous line of P3 now named P3K. This line was distributed to other workers including Cesar Milstein in Cambridge. Soon after the success with P3, Scharff and colleagues at Albert Einstein established MPC-11*in cul ture. Up to now continuous growth of human myeloma cells in vitro has remained a formidable problem.
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Medicine.
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Immunology.
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Allergy.
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Oncology.
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Potter, M.
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Warner, N. L.
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SpringerLink (Online service)
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Sponsored by the National Cancer Institute (NIH)
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