| Document Type
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BL
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| Record Number
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588617
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| Doc. No
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b417836
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| Title & Author
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Vascular disease : molecular biology and gene therapy protocols /\ edited by Andrew H. Baker.
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| Publication Statement
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Totowa, N.J. :: Humana Press,, ©1999.
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| Series Statement
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Methods in molecular medicine ;; 30
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| Page. NO
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1 online resource (xiv, 441 pages) :: illustrations.
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| ISBN
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0585246211
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: 9780585246215
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0896037312
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: 1592592473
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: 9781592592470
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: 9780896037311
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: 0896037312
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| Bibliographies/Indexes
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Includes bibliographical references and index.
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| Contents
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Pt.1. Genetics in vascular disease -- Pt.2. Isolation of genes expressed in vascular tissue -- Pt.3. Methods for mapping transcriptional start sites and measurement of promoter activity in vascular cells -- Pt.4. Molecular analysis of mRNA expression in vascular cells -- Pt.5. In vitro methods to express foreign genes in vascular cells -- Pt.6. In vivo vascular gene transfer protocols.
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| Abstract
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In Vascular Disease: Molecular Biology and Gene Therapy Protocols, Andrew Baker and a noted panel of expert investigators describe today's most powerful molecular methods for investigating the pathogenesis of vascular disease. These detailed, easy-to-follow techniques range from methods that have been used successfully to identify specific mutations involved in cardiovascular disorders, to those for transferring genes associated with cardiovascular disease into various vascular cell types by in vitro and in vivo routes. There are methods to identify novel genes and generate full-length cDNAs, to study gene transcription and promoter activity easily and effectively, and to ascertain precisely gene expression levels within the individual cell types in different pathophysiological conditions. Accurate methods to quantify apoptosis in both cultured cells and pathological specimens are also given. Vascular Disease: Molecular Biology and Gene Therapy Protocols offers today's vascular biologist and gene therapist an unprecedented ability to study the pathogenesis of vascular disease and readily to probe the potential for gene-based therapies. Powerful and productive, the techniques presented here operate across a wide range of exciting research areas, and promise spectacular therapeutic breakthroughs in the ongoing battle against vascular disease.
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| Subject
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Blood-vessels-- Diseases-- Molecular aspects, Laboratory manuals.
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Blood-vessels-- Diseases-- Gene therapy, Laboratory manuals.
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Blood-vessels-- Diseases-- Genetic aspects, Laboratory manuals.
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| Subject
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Vascular Diseases-- genetics.
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| Subject
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Molecular Biology-- methods.
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| LC Classification
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RC691.4.V36 1999eb
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| Added Entry
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Baker, Andrew H.
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